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IL-2 antibody

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Rabbit anti Mouse Interleukin-2:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-2

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AAM35B
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Rabbit anti Mouse Interleukin-2 antibody recognizes mouse Interleukin-2 (IL-2), a potent lymphoid cell growth factor which primarily acts on T lymphocytes, in response to antigenic or mitogenic stimulation.

IL-2 stimulates the growth/differentiation of monocytes, macrophages, B lymphocytes, NK cells, and lymphokine activated killer cells which are used in experimental cancer therapy.

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to mouse IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant mouse IL-2 (PMP38)
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM35B may be used in a direct ELISA or as a detection antibody in a sandwich ELISA with AAM35 as the capture reagent and PMP38 as the standard.

References for IL-2 antibody

  1. Smith, K.A. (1998) Interleukin-2: inception, impact, and implications.
    Science. 240: 1169-1176.

RRID
AB_2264652
UniProt
P04351
Entrez Gene
Il2
GO Terms
GO:0005125 cytokine activity
GO:0005134 interleukin-2 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0042104 positive regulation of activated T cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046013 regulation of T cell homeostatic proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050672 negative regulation of lymphocyte proliferation
GO:0050728 negative regulation of inflammatory response
GO:0051024 positive regulation of immunoglobulin secretion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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