IL-10 antibody

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Rabbit anti Mouse Interleukin-10

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM32 E FN WB 0.1 mg
Rabbit anti Mouse Interleukin-10 antibody recognizes mouse Interleukin-10 (IL-10), an immunosuppressive cytokine originally isolated from T helper type 2 cells (Th2), which is also secreted by B lymphocytes, macrophages, monocytes and keratinocytes.

IL-10 inhibits the synthesis of several cytokines including Tumour Necrosis Factor, Interferon-gamma and Interleukin-2 and -3, and has a wide range of biological properties, including inhibition of the macrophage and Th cell-mediated cytokine synthesis suppression of the delayed hypersensitivity response. IL-10 may initially be involved in the polarization of Th2 responses.

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant mouse IL-10
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P18893 Related reagents
Entrez Gene
Il10 Related reagents
GO Terms
GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus
GO:0042742 defense response to bacterium
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0030886 negative regulation of myeloid dendritic cell activation
GO:0032689 negative regulation of interferon-gamma production
GO:0032695 negative regulation of interleukin-12 production
GO:0032720 negative regulation of tumor necrosis factor production
GO:0044130 negative regulation of growth of symbiont in host
GO:0043193 positive regulation of gene-specific transcription
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0060670 branching involved in embryonic placenta morphogenesis
Regulatory
For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 0.02 0.04ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM32 may be used in an indirect ELISA with PMP21 as the standard.
Western Blotting
AAM32 may be used under either reducing or non-reducing Western blotting conditions with PMP21 as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Positive Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Mouse Interleukin-10 PMP21 10 µg E FN WB

Product Specific References

References for IL-10 antibody

  1. Ye, Q. et al. (2010) The relationship between collagen scaffold cross-linking agents and neutrophils in the foreign body reaction.
    Biomaterials. 31 (35): 9192-201.

Further Reading

  1. Barrat, F.J. et al. (2002) In vitro generation of interleukin 10-producing regulatory CD4(+) T cells is induced by immunosuppressive drugs and inhibited by T helper type 1 (Th1)- and Th2-inducing cytokines.
    J Exp Med. 195 (5): 603-16.