Rabbit anti Mouse Interleukin-10 antibody recognizes mouse Interleukin-10 (IL-10), an immunosuppressive cytokine originally isolated from T helper type 2 cells (Th2), which is also secreted by B lymphocytes, macrophages, monocytes and keratinocytes.
IL-10 inhibits the synthesis of several cytokines including Tumour Necrosis Factor, Interferon-gamma and Interleukin-2 and -3, and has a wide range of biological properties, including inhibition of the macrophage and Th cell-mediated cytokine synthesis suppression of the delayed hypersensitivity response. IL-10 may initially be involved in the polarization of Th2 responses.
- Target Species
- Product Form
- Purified IgG - lyophilized
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
- Antiserum Preparation
- Antisera to mouse IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant mouse IL-10.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of chronic inflammatory response to antigenic stimulus
defense response to bacterium
negative regulation of myeloid dendritic cell activation
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of tumor necrosis factor production
negative regulation of growth of symbiont in host
positive regulation of gene-specific transcription
positive regulation of MHC class II biosynthetic process
branching involved in embryonic placenta morphogenesis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-10 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- AAM32 may be used in an indirect ELISA with PMP21 as the standard.
- Western Blotting
- AAM32 may be used under either reducing or non-reducing Western blotting conditions with PMP21 as the positive control.
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Secondary Antibodies Available
Useful Reagents Available
Positive Isotype Controls Available
Product Specific References
References for IL-10 antibody
Ye, Q. et al. (2010) The relationship between collagen scaffold cross-linking agents and neutrophils in the foreign body reaction.
Biomaterials. 31 (35): 9192-201.
Barrat, F.J. et al. (2002) In vitro generation of interleukin 10-producing regulatory CD4(+) T cells is induced by immunosuppressive drugs and inhibited by T helper type 1 (Th1)- and Th2-inducing cytokines.
J Exp Med. 195 (5): 603-16.
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