IL-10 antibody

Rabbit anti Mouse Interleukin-10

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-10

Product Code Applications Pack Size List Price Your Price Qty
AAM32
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Mouse Interleukin-10 antibody recognizes mouse Interleukin-10 (IL-10), an immunosuppressive cytokine originally isolated from T helper type 2 cells (Th2), which is also secreted by B lymphocytes, macrophages, monocytes and keratinocytes.

IL-10 inhibits the synthesis of several cytokines including Tumour Necrosis Factor, Interferon-gamma and Interleukin-2 and -3, and has a wide range of biological properties, including inhibition of the macrophage and Th cell-mediated cytokine synthesis suppression of the delayed hypersensitivity response. IL-10 may initially be involved in the polarization of Th2 responses.

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant mouse IL-10.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 0.02 0.04ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM32 may be used in an indirect ELISA with PMP21 as the standard.
Western Blotting
AAM32 may be used under either reducing or non-reducing Western blotting conditions with PMP21 as the positive control.

Description Product Code Applications Pack Size List Price Your Price Quantity
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Mouse Interleukin-10 PMP21 E FN WB 10 µg loader
List Price Your Price
loader
Description Recombinant Mouse Interleukin-10

References for IL-10 antibody

  1. Ye, Q. et al. (2010) The relationship between collagen scaffold cross-linking agents and neutrophils in the foreign body reaction.
    Biomaterials. 31 (35): 9192-201.

Further Reading

  1. Barrat, F.J. et al. (2002) In vitro generation of interleukin 10-producing regulatory CD4(+) T cells is induced by immunosuppressive drugs and inhibited by T helper type 1 (Th1)- and Th2-inducing cytokines.
    J Exp Med. 195 (5): 603-16.

RRID
AB_324032
UniProt
P18893
Entrez Gene
Il10
GO Terms
GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus
GO:0042742 defense response to bacterium
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0030886 negative regulation of myeloid dendritic cell activation
GO:0032689 negative regulation of interferon-gamma production
GO:0032695 negative regulation of interleukin-12 production
GO:0032720 negative regulation of tumor necrosis factor production
GO:0044130 negative regulation of growth of symbiont in host
GO:0043193 positive regulation of gene-specific transcription
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0060670 branching involved in embryonic placenta morphogenesis
View more products with IL-10 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products