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IgM antibody

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Goat anti Mouse IgM:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

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Goat anti mouse IgM antibody recognises the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity is observed with human immunoglobulins.

Goat anti mouse IgM antibody has been cross absorbed against Mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.

Target Species
Mouse
Product Form
Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antiserum to mouse IgM was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on mouse IgM covalently linked to agarose.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse IgM paraproteins
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

References for IgM antibody

  1. Kerr, K. et al. (2010) Inflammatory cytokine responses in a pregnant mouse model of Chlamydophila abortus infection.
    Vet Microbiol. 144 (3-4): 392-8.
  2. Kamat, M.M. et al. (2016) Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy.
    Biol Reprod. 95 (3): 68.
  3. Monzo, H.J. et al. (2017) Insulin promotes cell migration by regulating PSA-NCAM.
    Exp Cell Res. 355 (1): 26-39.
  4. Desancé, M. et al. (2018) Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy.
    Int J Mol Sci.19 (2): 537.
  5. Penadés, M. et al. (2018) Long-term implications of feed energy source in different genetic types of reproductive rabbit females. II. Immunologic status.
    Animal. 12 (9): 1877-85.
  6. Penadés, M. et al. (2019) Early deviations in performance, metabolic and immunological indicators affect stayability in rabbit females.
    Animal. : 1-10.

RRID
AB_619870
UniProt
P01872
P01873
Entrez Gene
Ighm
Ighm
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0003823 antigen binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0042571 immunoglobulin complex, circulating
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
GO:0016021 integral to membrane

102002

155621 158058 158297 158622 159911 160534

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