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IgM antibody

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Goat anti Mouse IgM:Biotin

Goat anti Mouse IgM:FITC

Goat anti Mouse IgM:HRP

Goat anti Mouse IgM

Goat anti Mouse IgM:RPE

Goat anti Mouse IgM:Texas Red®

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
102008
Datasheet
E F IB IF
MSDS
1 mg loader

102002
Datasheet
F
MSDS
1 mg loader

102005
Datasheet
C E IB
MSDS
1 ml loader

102001
Datasheet
E F IF
MSDS
1 mg loader

102009
Datasheet
F
MSDS
0.5 mg loader

102007
Datasheet
F
MSDS
1 mg loader

Goat anti mouse IgM antibody recognises the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity is observed with human immunoglobulins.

Goat anti mouse IgM antibody has been cross absorbed against Mouse IgG1, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified Ig conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified Ig conjugated to R. Phycoerythrin (RPE) - liquid
Product Form
Purified Ig conjugated to Texas Red® (TXRD) - Liquid
Preparation
Purified Ig prepared by affinity chromatography on IgM covalently linked to agarose
Antiserum Preparation
Purified Ig prepared by affinity chromatography on IgM covalently linked to agarose
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium azide
Preservative Stabilisers
50% Glycerol
Preservative Stabilisers
None present
Preservative Stabilisers
0.1% Sodium azide
Stabilizing agent
Preservative Stabilisers
0.1% Sodium azide
Immunogen
Mouse IgM paraproteins
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
+4ºC, -20ºC if preferred
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
18 months from date of despatch
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P01872 Related reagents
P01873 Related reagents
Entrez Gene
Ighm Related reagents
Ighm Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0003823 antigen binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0042571 immunoglobulin complex, circulating
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
GO:0016021 integral to membrane
Acknowledgements
Texas Red® is a registered trademark of Molecular Probes, Inc.
Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunoblotting
Immunofluorescence
Flow Cytometry 1/100
ELISA 1/4,000 1/8,000
Immunoblotting
Immunohistology - Frozen
ELISA
Flow Cytometry
Immunofluorescence
Flow Cytometry
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgM antibody

  1. Kerr, K. et al. (2010) Inflammatory cytokine responses in a pregnant mouse model of Chlamydophila abortus infection.
    Vet Microbiol. 144 (3-4): 392-8.
  2. Ferrian, S. et al. (2012) Effect of high temperature on blood lymphocyte populations in two different genetic rabbit lines.
    Proceedings 10 th World Rabbit Congress 1169-73
  3. Kamat, M.M. et al. (2016) Changes in Myeloid Lineage Cells in the Uterus and Peripheral Blood of Dairy Heifers During Early Pregnancy.
    Biol Reprod. 95 (3): 68.
  4. Monzo, H.J. et al. (2017) Insulin promotes cell migration by regulating PSA-NCAM.
    Exp Cell Res. 355 (1): 26-39.
  5. Desancé, M. et al. (2018) Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy.
    Int J Mol Sci. 19 (2)Feb 10 [Epub ahead of print].
  6. Penadés, M. et al. (2018) Long-term implications of feed energy source in different genetic types of reproductive rabbit females. II. Immunologic status.
    Animal. 12 (9): 1877-85.
  7. Penadés, M. et al. (2019) Early deviations in performance, metabolic and immunological indicators affect stayability in rabbit females.
    Animal. : 1-10.
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