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|Goat anti Mouse IgG2c antibody recognizes mouse IgG2c, otherwise known as the Igh 1b allele of IgG2a. Goat anti Mouse IgG2c antibody is assessed by ELISA and immunoelectrophoresis and has been shown to react with IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice and does not react with L-chains or other IgG subclasses.|
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography
- Antiserum Preparation
- Antisera to mouse IgG2c were raised by repeated immunisations of goats with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
- Native mouse IgG2c.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 1x106 cells in 100ul.
References for IgG2c antibody
Jalili, R.B. et al. (2010) Local Expression of Indoleamine 2,3 Dioxygenase in Syngeneic Fibroblasts Significantly Prolongs Survival of an Engineered Three-Dimensional Islet Allograft.
Diabetes 59: 2219-27
Arnold, I.C. et al. (2011) The C-terminally encoded, MHC class II-restricted T cell antigenicity of the Helicobacter pylori virulence factor CagA promotes gastric preneoplasia.
J Immunol. 186: 6165-72.
Paes, W. et al. (2016) Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice.
Vaccine. 34 (35): 4123-31.
Carroll, E.C. et al. (2016) The Vaccine Adjuvant Chitosan Promotes Cellular Immunity via DNA Sensor cGAS-STING-Dependent Induction of Type I Interferons.
Immunity. 44 (3): 597-608.
Zouggari, Y. et al. (2013) B lymphocytes trigger monocyte mobilization and impair heart function after acute myocardial infarction.
Nat Med. 19 (10): 1273-80.
Müller-Winkler, J. et al. (2021) Critical requirement for BCR, BAFF, and BAFFR in memory B cell survival.
J Exp Med. 218 (2) [Epub ahead of print].
McEntee, C.P. et al. (2020) Type I IFN signalling is required for cationic adjuvant formulation (CAF)01-induced cellular immunity and mucosal priming.
Vaccine. 38 (3): 635-43.
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