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IgG IgA IgM antibody

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Goat anti Mouse IgG/A/M:Alk. Phos.

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG IgA IgM

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Goat anti Mouse IgG/A/M (Human Adsorbed) antibody recognizes all subclasses of murine immunoglobulin and has been adsorbed against human, bovine and hamster immunoglobulins.

Target Species
Mouse
Product Form
Purified IgG conjugated to alkaline phosphatase - liquid
Antiserum Preparation
Antisera to mouse immunoglobulins were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
0.05M TRIS Chloride
0.15M NaCl
0.001M MgCl2
0.0001M ZnCl2
50% (v/v) Glycerol; pH8.0
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Mouse IgG purified from normal mouse serum, mouse IgA and IgM purified from ascitic fluid.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/3000
Immunohistology - Frozen
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for IgG IgA IgM antibody

  1. Bergmeier, L.A. et al. (2005) Mucosal alloimmunization elicits T-cell proliferation, CC chemokines, CCR5 antibodies and inhibition of simian immunodeficiency virus infectivity.
    J Gen Virol. 86: 2231-8.
  2. Peters, B. et al. (2004) Effect of heterosexual intercourse on mucosal alloimmunisation and resistance to HIV-1 infection.
    Lancet. 363: 518-24.
  3. Bartlomiejczyk, M.A. et al. (2014) Interaction of lectin pathway of complement-activating pattern recognition molecules with mycobacteria.
    Clin Exp Immunol. 178 (2): 310-9.
  4. Kubelkova, K. et al. (2021) Early infection-induced natural antibody response.
    Sci Rep. 11 (1): 1541.
  5. Grandoni, F. et al. (2017) Characterization of leukocyte subsets in buffalo (Bubalus bubalis.) with cross-reactive monoclonal antibodies specific for bovine MHC class I and class II molecules and leukocyte differentiation molecules.
    Dev Comp Immunol. 74: 101-109.

Flow Cytometry

RRID
AB_321852
UniProt
P01867
P01865
P01864
P01863
P01869
P01868
P03987
P01872
P01873
P01878
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
Ighm
Ighm
Igh-VJ558
AI324046
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway

STAR87A

147952 150602 152952

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