IgG IgA IgM antibody

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Goat anti Mouse IgG/A/M (H/L):Biotin

Goat anti Mouse IgG/A/M (H/L):FITC

Goat anti Mouse IgG/A/M (H/L):HRP

Goat anti Mouse IgG/A/M (H/L)

Goat anti Mouse IgG/A/M (H/L):RPE

Goat anti Mouse IgG/A/M (H/L):Texas Red®

Goat anti Mouse IgG/A/M (H/L):Bgal

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
101008	E IB			1 mg
101002	F			1 mg
101005	E WB			1 ml
101001	E F IB			2 mg
101009	F			0.5 mg
101007	F			1 mg
101006	E			1 ml

Goat anti Mouse IgG/A/M reacts with the heavy and light chains of all major classes of mouse immunoglobulin.

The antibody has been adsorbed against human serum to minimise cross-reactivity with human immunoglobulins.

Product Details

Target Species

Mouse

Product Form

Ig Fraction conjugated to Biotin - liquid

Product Form

Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid

Product Form

Purified IgG conjugated to Texas Red^® - liquid

Product Form

Ig Fraction conjugated to Beta Galactosidase - liquid

Preparation

Purified IgG prepared by affinity chromatography on pooled mouse IgM, IgG and IgA covalently linked to agarose

Antiserum Preparation

Antisera to Mouse IgG, IgA and IgM were raised by repeated immunisation of goats with pooled mouse IgG, IgA and IgM paraproteins. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Borate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1%	Sodium Azide (NaN₃)

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)

Preservative Stabilisers

50% Glycerol

Preservative Stabilisers

None present.

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)
Stabilizing agent

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)

50% Glycerol

Immunogen

Purified mouse IgG, IgA and IgM.

Approx. Protein Concentrations

Ig concentration 0.5 mg/ml

Approx. Protein Concentrations

IgG concentration 1mg/ml

Approx. Protein Concentrations

IgG concentration 1mg/ml

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: Please see label for expiry date.
Shelf Life: 12 months from date of despatch.
Shelf Life: Please see label for expiry date
Shelf Life: Please see label for expiry date.
Shelf Life: Please see label for expiry date.
Shelf Life: Please see label for expiry date.
Shelf Life: Please see label for expiry date

More Information

UniProt: P01864 Related reagents; P01872 Related reagents; P03987 Related reagents; P01873 Related reagents; P01868 Related reagents; P01865 Related reagents; P01869 Related reagents; P01878 Related reagents; P01867 Related reagents; P01863 Related reagents; P01844 Related reagents; P01843 Related reagents; P01845 Related reagents; P01834 Related reagents
Entrez Gene: IGKC Related reagents; LOC433053 Related reagents; Iglc2 Related reagents; Iglc3 Related reagents; Igh-1a Related reagents; Igh-1a Related reagents; Igh-1a Related reagents; Ighg2b Related reagents; Ighg1 Related reagents; Ighg1 Related reagents; Ighm Related reagents; Ighm Related reagents; Igh-VJ558 Related reagents; AI324046 Related reagents
GO Terms: GO:0005515 protein binding; GO:0003823 antigen binding; GO:0005576 extracellular region; GO:0006958 complement activation, classical pathway; GO:0045087 innate immune response; GO:0009897 external side of plasma membrane; GO:0019815 B cell receptor complex; GO:0002455 humoral immune response mediated by circulating immunoglobulin; GO:0019731 antibacterial humoral response; GO:0042571 immunoglobulin complex, circulating; GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0001788 antibody-dependent cellular cytotoxicity; GO:0001798 positive regulation of type IIa hypersensitivity; GO:0001812 positive regulation of type I hypersensitivity; GO:0006910 phagocytosis, recognition; GO:0006911 phagocytosis, engulfment; GO:0050766 positive regulation of phagocytosis; GO:0000187 activation of MAPK activity; GO:0004888 transmembrane receptor activity; GO:0019882 antigen processing and presentation; GO:0030890 positive regulation of B cell proliferation; GO:0043410 positive regulation of MAPKKK cascade; GO:0045022 early endosome to late endosome transport; GO:0045807 positive regulation of endocytosis; GO:0048471 perinuclear region of cytoplasm; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0050853 B cell receptor signaling pathway
Acknowledgements: Texas Red^® is a registered trademark of Molecular Probes, Inc..
Regulatory: For research purposes only

Applications of IgG IgA IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/2000	1/10,000
Immunoblotting
Flow Cytometry		1/5	1/20
ELISA		1/4000	1/8000
Western Blotting		1/2000	1/10,000
ELISA
Flow Cytometry
Immunoblotting
Flow Cytometry		1/20	1/50
Flow Cytometry		1/5	1/20
ELISA		1/100	1/500

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E

Product Specific References

References for IgG IgA IgM antibody

Ding, G. et al. (2010) Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla.
J Cell Physiol. 22: 415-22.
Shao, L. et al. (2015) Comparative In Vitro and In Vivo Studies of Porcine Rotavirus G9P[13] and Human Rotavirus Wa G1P[8].
J Virol. 90 (1): 142-51.

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