IgG antibody

100% Secure

Rabbit F(ab')2 anti Mouse IgG

Rabbit F(ab')2 anti Mouse IgG:DyLight®549

Rabbit F(ab')2 anti Mouse IgG:DyLight®649

Rabbit F(ab')2 anti Mouse IgG:DyLight®488

Rabbit F(ab')2 anti Mouse IgG:Dylight®800

Rabbit F(ab')2 anti Mouse IgG:Dylight®405

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR8B C E P 1 mg
STAR8D549GA F IF 0.1 mg
STAR8D649GA F IF 0.1 mg
STAR8D488GA F IF 0.1 mg
STAR8D800GA F IF WB 0.1 mg
STAR8D405GA F IF 0.1 mg
Rabbit F(ab')2 anti Mouse IgG antibody recognizes all subclasses of mouse IgG. Cross reactivity with rat IgG is expected. Cross reactivity with human serum proteins has been minimised by solid phase adsorbtion.

STAR8B is suitable as a bridge in a PAP / APAAP complex. Peroxidase anti-Peroxidase (PAP) complexes are formed by antibodies raised towards Horse Radish Peroxidase (HRP), binding to HRP molecules. Such complexes are used as signal amplification reagents in immunochemical staining techniques. It must be emphasised that the primary antibody must be raised in the same species as the antibodies used to form the PAP complex, so that a secondary "linker" antibody can be used to "bridge" the primary antibody to the PAP complex.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to DyLight®549 - liquid
Product Form
Purified IgG conjugated to DyLight®649 - liquid
Product Form
Purified IgG conjugated to DyLight®488 - liquid
Product Form
Purified IgG conjugated to DyLight®800 - liquid
Product Form
Purified IgG conjugated to DyLight®405 - liquid
Preparation
Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments.
Preparation
Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments.
Preparation
Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments.
Preparation
Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments
Preparation
Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments
Preparation
Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Whole mouse IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch

More Information

UniProt
P01869 Related reagents
P01865 Related reagents
P03987 Related reagents
P01867 Related reagents
P01868 Related reagents
P01864 Related reagents
P01863 Related reagents
Entrez Gene
Igh-1a Related reagents
Igh-1a Related reagents
Igh-1a Related reagents
Ighg2b Related reagents
Ighg1 Related reagents
Ighg1 Related reagents
AI324046 Related reagents
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Immunohistology - Frozen 1/100 1/500
Immunohistology - Paraffin 1/100 1/500
Flow Cytometry 1/200 1/500
Immunofluorescence 1/200 1/500
Flow Cytometry 1/100 1/500
Immunofluorescence 1/100 1/500
Flow Cytometry Neat 1/25
Immunofluorescence
Flow Cytometry
Immunofluorescence
Western Blotting 1/10000 1/50000
Flow Cytometry 1/20 1/200
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for IgG antibody

  1. Seehafer, S.S. et al. (2011) Immunosuppression alters disease severity in juvenile Batten disease mice.
    J Neuroimmunol. 230 (1-2): 169-72.
  2. Motallebzadeh, R. et al. (2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.
    FASEB J. 26 (1): 51-62.
  3. Niven, J. et al. (2015) S100B Up-Regulates Macrophage Production of IL1β and CCL22 and Influences Severity of Retinal Inflammation.
    PLoS One. 10 (7): e0132688.