IgG antibody

Sheep anti Mouse IgG (H/L) antibody has been shown to cross-react with murine IgA and IgM.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Bovine Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Antiserum Preparation
- Antisera to mouse IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Polyclonal mouse IgG.
- Approx. Protein Concentrations
- IgG concentration 1mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01869
- P01837
- P01867
- P01724
- P01865
- P01844
- P01864
- P01868
- P01843
- P01863
- P01845
- P03987
- Entrez Gene
- Iglv1
- Igk-C
- LOC433053
- Iglc2
- Iglc3
- Igh-1a
- Igh-1a
- Igh-1a
- Ighg2b
- Ighg1
- Ighg1
- AI324046
- GO Terms
- GO:0003823 antigen binding
- GO:0005886 plasma membrane
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0009897 external side of plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0019731 antibacterial humoral response
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019815 B cell receptor complex
- GO:0050766 positive regulation of phagocytosis
- GO:0042571 immunoglobulin complex, circulating
- Regulatory
- For research purposes only
Applications of IgG antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/5000 | 1/10000 | |
Immunohistology - Frozen 1 | 1/40 | 1/80 | |
Immunohistology - Paraffin | |||
Western Blotting | 1/5000 | 1/15000 |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml |
Product Specific References
References for IgG antibody
-
Hsiao, Y.W. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells.
Vet Immunol Immunopathol. 87 (1-2): 19-27. -
Baur, K. etal (2010) Immediate-Early Expression of a Recombinant Antigen by Modified Vaccinia Virus Ankara Breaks the Immunodominance of Strong Vector-Specific B8R Antigen in Acute and Memory CD8 T-Cell Responses.
J Virol. 84: 8743-52. -
Pacheco, J.M. et al. (2010) IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination.
Clin Vaccine Immunol. 17: 550-8. -
Kim HR et al. (2017) In vitro inflammatory effects of polyhexamethylene biguanide through NF-κB activation in A549 cells.
Toxicol In Vitro. 38: 1-7. -
Volkmann, A. et al. (2020) Recombinant Modified Vaccinia Virus Ankara (mva) Filovirus Vaccine
US Patent Application: US 2020/0268872 A1
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