IgG antibody

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Sheep anti Mouse IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAC10P C* E WB 1 mg
Sheep anti Mouse IgG (H/L) antibody recognizes mouse IgG heavy and light chains.

Sheep anti Mouse IgG (H/L) antibody has been shown to cross-react with murine IgA and IgM.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Polyclonal mouse IgG.
Approx. Protein Concentrations
IgG concentration 1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
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GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/10000
Immunohistology - Frozen 1 1/40 1/80
Immunohistology - Paraffin
Western Blotting 1/5000 1/15000
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG antibody

  1. Hsiao, Y.W. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells.
    Vet Immunol Immunopathol. 87 (1-2): 19-27.
  2. Baur, K. etal (2010) Immediate-Early Expression of a Recombinant Antigen by Modified Vaccinia Virus Ankara Breaks the Immunodominance of Strong Vector-Specific B8R Antigen in Acute and Memory CD8 T-Cell Responses.
    J Virol. 84: 8743-52.
  3. Pacheco, J.M. et al. (2010) IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination.
    Clin Vaccine Immunol. 17: 550-8.
  4. Kim HR et al. (2017) In vitro inflammatory effects of polyhexamethylene biguanide through NF-κB activation in A549 cells.
    Toxicol In Vitro. 38: 1-7.