IgG antibody

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Sheep anti Mouse IgG (H/L):HRP

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AAC10P Datasheet	C* E WB SDS	1 mg	Log in		Get Quote

Sheep anti Mouse IgG (H/L) antibody recognizes mouse IgG heavy and light chains.

Sheep anti Mouse IgG (H/L) antibody has been shown to cross-react with murine IgA and IgM.

Product Details

Target Species

Mouse

Species Cross-Reactivity

Target Species	Cross Reactivity
Human
Bovine
Rabbit

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Antiserum Preparation

Antisera to mouse IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.01% Thiomersal

Immunogen

Polyclonal mouse IgG.

Approx. Protein Concentrations

IgG concentration 1mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P01869; P01837; P01867; P01724; P01865; P01844; P01864; P01868; P01843; P01863; P01845; P03987
Entrez Gene: Iglv1; Igk-C; LOC433053; Iglc2; Iglc3; Igh-1a; Igh-1a; Igh-1a; Ighg2b; Ighg1; Ighg1; AI324046
GO Terms: GO:0003823 antigen binding; GO:0009897 external side of plasma membrane; GO:0019815 B cell receptor complex; GO:0002455 humoral immune response mediated by circulating immunoglobulin; GO:0019731 antibacterial humoral response; GO:0042571 immunoglobulin complex, circulating; GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0001788 antibody-dependent cellular cytotoxicity; GO:0001798 positive regulation of type IIa hypersensitivity; GO:0001812 positive regulation of type I hypersensitivity; GO:0006910 phagocytosis, recognition; GO:0006911 phagocytosis, engulfment; GO:0006958 complement activation, classical pathway; GO:0050766 positive regulation of phagocytosis
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/5000	1/10000
Immunohistology - Frozen ¹	1/40	1/80
Immunohistology - Paraffin
Western Blotting	1/5000	1/15000

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
AbGUARD® HRP Stabilizer Plus	BUF052A	C E P WB	100 ml	Log in	Get Quote
AbGUARD® HRP Stabilizer Plus	BUF052B	C E P WB	500 ml	Log in	Get Quote
AbGUARD® HRP Stabilizer Plus	BUF052C	C E P WB	1000 ml	Log in	Get Quote
TMB Signal+	BUF054A	E	100 ml	Log in	Get Quote
TMB Core	BUF056A	E	100 ml	Log in	Get Quote
TMB Core+	BUF062A	E	100 ml	Log in	Get Quote

Product Specific References

References for IgG antibody

Hsiao, Y.W. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells.
Vet Immunol Immunopathol. 87 (1-2): 19-27.
Baur, K. etal (2010) Immediate-Early Expression of a Recombinant Antigen by Modified Vaccinia Virus Ankara Breaks the Immunodominance of Strong Vector-Specific B8R Antigen in Acute and Memory CD8 T-Cell Responses.
J Virol. 84: 8743-52.
Pacheco, J.M. et al. (2010) IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination.
Clin Vaccine Immunol. 17: 550-8.
Kim HR et al. (2017) In vitro inflammatory effects of polyhexamethylene biguanide through NF-κB activation in A549 cells.
Toxicol In Vitro. 38: 1-7.
Volkmann, A. et al. (2020) Recombinant Modified Vaccinia Virus Ankara (mva) Filovirus Vaccine
US Patent Application: US 2020/0268872 A1
Park, J.S. et al. (2019) Polyhexamethylene guanidine phosphate-induced ROS-mediated DNA damage caused cell cycle arrest and apoptosis in lung epithelial cells.
J Toxicol Sci. 44 (6): 415-424.

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