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IgG antibody

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Sheep anti Mouse IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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C * E WB 1 mg loader
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Sheep anti Mouse IgG (H/L) antibody recognizes mouse IgG heavy and light chains.

Sheep anti Mouse IgG (H/L) antibody has been shown to cross-react with murine IgA and IgM.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Polyclonal mouse IgG.
Approx. Protein Concentrations
IgG concentration 1mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/10000
Immunohistology - Frozen 1 1/40 1/80
Immunohistology - Paraffin
Western Blotting 1/5000 1/15000
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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TMB Core BUF056A E 100 ml
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TMB Core+ BUF062A E 100 ml
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Description TMB Core+

References for IgG antibody

  1. Hsiao, Y.W. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells.
    Vet Immunol Immunopathol. 87 (1-2): 19-27.
  2. Baur, K. etal (2010) Immediate-Early Expression of a Recombinant Antigen by Modified Vaccinia Virus Ankara Breaks the Immunodominance of Strong Vector-Specific B8R Antigen in Acute and Memory CD8 T-Cell Responses.
    J Virol. 84: 8743-52.
  3. Pacheco, J.M. et al. (2010) IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination.
    Clin Vaccine Immunol. 17: 550-8.
  4. Kim HR et al. (2017) In vitro inflammatory effects of polyhexamethylene biguanide through NF-κB activation in A549 cells.
    Toxicol In Vitro. 38: 1-7.
  5. Volkmann, A. et al. (2020) Recombinant Modified Vaccinia Virus Ankara (mva) Filovirus Vaccine
    US Patent Application: US 2020/0268872 A1
  6. Park, J.S. et al. (2019) Polyhexamethylene guanidine phosphate-induced ROS-mediated DNA damage caused cell cycle arrest and apoptosis in lung epithelial cells.
    J Toxicol Sci. 44 (6): 415-424.

RRID
AB_321929
UniProt
P01869
P01837
P01867
P01724
P01865
P01844
P01864
P01868
P01843
P01863
P01845
P03987
Entrez Gene
Iglv1
Igk-C
LOC433053
Iglc2
Iglc3
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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