IgG antibody
* Stain-free gels can be used for total protein normalization in place of or in addition to housekeeping proteins (HKP)
StarBright Blue 700 conjugated Goat anti mouse IgG antibody (12004158) used to detect bound mouse antibodies in western blotting
Image caption:
Analyses of FMR1, CTCF and MAX expression in the thymus and thymic epithelial cells.
Western blots for FMPR and GAPDH on thymic extracts. Six bands were recognized by the anti-FMRP antibody.
From: Thomas S, Fayet OM, Truffault F, Fadel E, Provost B, Hamza A, Berrih-Aknin S, Bonnefont JP, Le Panse R.
Altered expression of fragile X mental retardation-1 (FMR1) in the thymus in autoimmune myasthenia gravis.
J Neuroinflammation. 2021 Nov 17;18(1):270.
doi: 10.1186/s12974-021-02311-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
StarBright Blue 700 conjugated Goat anti Mouse IgG antibody (12004158) used to label bound mouse antibodies by western blotting.
Image caption:
LOF[IM] mutations disrupt crosslinking between RcsF and plasmid-encoded IgaA-FLAG.
Immunoblot analysis of in vivo formaldehyde crosslinked samples and the validation of the RcsF/IgaA crosslinking band. The membrane was probed with mouse α-FLAG and rabbit α-RcsF antibodies. To facilitate simultaneous detection, the membrane was probed with Bio-Rad Anti-Mouse IgG StarBright Blue 700 and Anti-Rabbit IgG StarBright Blue 520 secondary antibodies. Membranes were visualized using the ChemiDoc MP Imaging System (Bio-Rad). Top panels are the black-and-white images of the single-channel fluorescent images; the bottom panel is the colored overlay of the single-channel images. The immunoblot quantification is not presented since no RcsF/IgaA bands were detected in LOF[IM] mutant samples.
From: Lach SR, Kumar S, Kim S, Im W, Konovalova A (2023)
Conformational rearrangements in the sensory RcsF/OMP complex mediate signal transduction across the bacterial cell envelope.
PLoS Genet 19(1): e1010601.
doi: 10.1371/journal.pgen.1010601
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB F ResetGoat ANTI-MOUSE IgG StarBright™ Blue 700
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Antibody Quality and Performance Guarantee
| StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores. StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot. Features and Benefits Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm) Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies Goat Anti-Mouse IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, rabbit and rat. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to StarBright Blue 700 - lyophilized
- Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
- Antiserum Preparation
- Antiserum to Mouse IgG was raised by repeated immunisation of Goats with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- >80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Blue 700 470 700 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Western Blotting | |
1/2,500 | 1/5,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing. - Instructions For Use
-
12004159, 12004158
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG antibody
-
Dunbar, K. et al. (2021) IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling.
Life Sci Alliance. 4 (2)Dec 23 [Epub ahead of print]. -
Dunbar, K. et al. (2021) FAM83F regulates canonical Wnt signalling through an interaction with CK1α.
Life Sci Alliance. 4 (2)Dec 24 [Epub ahead of print]. -
Holoch, D. et al. (2021) A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals.
Nat Genet. 53 (12): 1686-97. -
Thomas, S. et al. (2021) Altered expression of fragile X mental retardation-1 (FMR1) in the thymus in autoimmune myasthenia gravis.
J Neuroinflammation. 18 (1): 270. -
Han, H. et al. (2021) Loss of aryl hydrocarbon receptor suppresses the response of colonic epithelial cells to IL22 signaling by upregulating SOCS3.
Am J Physiol Gastrointest Liver Physiol. Nov 10 [Epub ahead of print]. -
Meissner, M.E. et al. (2021) Differential Activity of APOBEC3F, APOBEC3G, and APOBEC3H in the Restriction of HIV-2.
J Mol Biol. 434 (2): 167355. -
Goad, D.W.et al. (2021) Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
Molecular Therapy - Oncolytics. Nov 27 [Epub ahead of print]. -
Baumann, C. et al. (2021) Changes in chromatin accessibility landscape and histone H3 core acetylation during valproic acid-induced differentiation of embryonic stem cells.
Epigenetics Chromatin. 14 (1): 58.
View The Latest Product References
-
Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
Life Sci Alliance. 5(3):e202101185. -
Cejas, R.B. et al. (2022) Impact of DYRK1A Expression on TNNT2 Splicing and Daunorubicin Toxicity in Human iPSC-Derived Cardiomyocytes.
Cardiovasc Toxicol. May 21 [Epub ahead of print]. -
Lach, S.R. et al. (2023) Conformational rearrangements in the sensory RcsF/OMP complex mediate signal transduction across the bacterial cell envelope.
PLoS Genet. 19 (1): e1010601. -
Chae, S.A. et al. (2023) Exercise enhances placental labyrinth trophoblast development by activation of PGC-1α and FNDC5/irisin.
Biol Reprod. Nov 2 :ioad151. [Epub ahead of print]. -
Ferreira-Santos, L. et al. (2023) Neuraminidase-induced externalization of phosphatidylserine activates ADAM17 and impairs insulin signaling in endothelial cells.
Am J Physiol Heart Circ Physiol. Nov 24 [Epub ahead of print]. -
Hibino, K. et al. (2024) Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes.
Nat Commun. 15 (1): 7152. -
Ramzan, F. et al. (2024) Fatty links between multisystem proteinopathy and small VCP-interacting protein.
Cell Death Discov. 10 (1): 358. -
Solís, C. et al. (2023) Cardiomyocyte external mechanical unloading activates modifications of α-actinin differently from sarcomere-originated unloading.
FEBS J. 290 (22): 5322-5339.
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