IgG antibody

Goat ANTI-MOUSE IgG StarBright™ Blue 700

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

Product Code Applications Pack Size List Price Your Price Qty
12004158
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 400 µl loader
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12004159
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 80 µl loader
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StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores.

StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.

Features and Benefits

Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot
Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm)
Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies

Goat Anti-Mouse IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, rabbit and rat.

Target Species
Mouse
Product Form
Purified IgG conjugated to StarBright Blue 700 - lyophilized
Reconstitution
Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
Antiserum Preparation
Antiserum to Mouse IgG was raised by repeated immunisation of Goats with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
>80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Blue 700 470 700
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting 1/2,500 1/5,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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Description Goat ANTI-RABBIT IgG StarBright™ Blue 700
Goat ANTI-RABBIT IgG StarBright™ Blue 700 12004162 WB 80 µl loader
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Description Goat ANTI-RABBIT IgG StarBright™ Blue 700
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Description ANTI-TUBULIN hFAB™ Rhodamine Antibody
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References for IgG antibody

  1. Dunbar, K. et al. (2021) IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling.
    Life Sci Alliance. 4 (2)Dec 23 [Epub ahead of print].
  2. Dunbar, K. et al. (2021) FAM83F regulates canonical Wnt signalling through an interaction with CK1α.
    Life Sci Alliance. 4 (2)Dec 24 [Epub ahead of print].
  3. Holoch, D. et al. (2021) A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals.
    Nat Genet. 53 (12): 1686-97.
  4. Thomas, S. et al. (2021) Altered expression of fragile X mental retardation-1 (FMR1) in the thymus in autoimmune myasthenia gravis.
    J Neuroinflammation. 18 (1): 270.
  5. Han, H. et al. (2021) Loss of aryl hydrocarbon receptor suppresses the response of colonic epithelial cells to IL22 signaling by upregulating SOCS3.
    Am J Physiol Gastrointest Liver Physiol. Nov 10 [Epub ahead of print].
  6. Meissner, M.E. et al. (2021) Differential Activity of APOBEC3F, APOBEC3G, and APOBEC3H in the Restriction of HIV-2.
    J Mol Biol. 434 (2): 167355.
  7. Goad, D.W.et al. (2021) Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
    Molecular Therapy - Oncolytics. Nov 27 [Epub ahead of print].
  8. Baumann, C. et al. (2021) Changes in chromatin accessibility landscape and histone H3 core acetylation during valproic acid-induced differentiation of embryonic stem cells.
    Epigenetics Chromatin. 14 (1): 58.
  9. View The Latest Product References
  10. Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
    Life Sci Alliance. 5(3):e202101185.
  11. Cejas, R.B. et al. (2022) Impact of DYRK1A Expression on TNNT2 Splicing and Daunorubicin Toxicity in Human iPSC-Derived Cardiomyocytes.
    Cardiovasc Toxicol. May 21 [Epub ahead of print].
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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