IGF-I antibody
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Goat anti Mouse Insulin-Like Growth Factor I antibody recognizes mouse insulin-like growth factor-1 (IGF-1).
IGF-1 stimulates the proliferation of a range of cell types including bone, cartilage tissue and muscle.
IGF-1 stimulates the proliferation of a range of cell types including bone, cartilage tissue and muscle.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse IGF-1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None Present
- Immunogen
- Recombinant mouse IGF-1 (PMP44)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P05017 Related reagents
- Entrez Gene
- Igf1 Related reagents
- GO Terms
- GO:0001649 osteoblast differentiation
- GO:0001932 regulation of protein phosphorylation
- GO:0001974 blood vessel remodeling
- GO:0005159 insulin-like growth factor receptor binding
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0008083 growth factor activity
- GO:0008285 negative regulation of cell proliferation
- GO:0010001 glial cell differentiation
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0046326 positive regulation of glucose import
- GO:0021940 positive regulation of cerebellar granule cell precursor proliferation
- GO:0030104 water homeostasis
- GO:0030879 mammary gland development
- GO:0031017 exocrine pancreas development
- GO:0032878 regulation of establishment or maintenance of cell polarity
- GO:0035264 multicellular organism growth
- GO:0045725 positive regulation of glycogen biosynthetic process
- GO:0048009 insulin-like growth factor receptor signaling pathway
- GO:0048286 lung alveolus development
- GO:0048468 cell development
- GO:0048754 branching morphogenesis of a tube
- GO:0050650 chondroitin sulfate proteoglycan biosynthetic process
- GO:0051897 positive regulation of protein kinase B signaling cascade
- GO:0060426 lung vasculature development
- GO:0060463 lung lobe morphogenesis
- GO:0060509 Type I pneumocyte differentiation
- GO:0060510 Type II pneumocyte differentiation
- GO:0060527 prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis
- GO:0060736 prostate gland growth
- GO:0060741 prostate gland stromal morphogenesis
- GO:0060766 negative regulation of androgen receptor signaling pathway
- GO:0070373 negative regulation of ERK1 and ERK2 cascade
- Regulatory
- For research purposes only
Applications of IGF-I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 12.0 | 14.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
