IGF-I antibody

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Goat anti Mouse Insulin-Like Growth Factor I

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AAM30 Datasheet	E FN WB SDS	0.1 mg	Log in		Get Quote

Goat anti Mouse Insulin-Like Growth Factor I antibody recognizes mouse insulin-like growth factor-1 (IGF-1).

IGF-1 stimulates the proliferation of a range of cell types including bone, cartilage tissue and muscle.

Product Details

Target Species: Mouse
Product Form: Purified IgG - lyophilized
Reconstitution: Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation: Antisera to mouse IGF-1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None Present
Carrier Free: Yes
Immunogen: Recombinant mouse IGF-1 (PMP44)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 6 months from date of despatch

More Information

UniProt: P05017
Entrez Gene: Igf1
GO Terms: GO:0001649 osteoblast differentiation; GO:0001932 regulation of protein phosphorylation; GO:0001974 blood vessel remodeling; GO:0005159 insulin-like growth factor receptor binding; GO:0005179 hormone activity; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0008083 growth factor activity; GO:0008285 negative regulation of cell proliferation; GO:0010001 glial cell differentiation; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0046326 positive regulation of glucose import; GO:0021940 positive regulation of cerebellar granule cell precursor proliferation; GO:0030104 water homeostasis; GO:0030879 mammary gland development; GO:0031017 exocrine pancreas development; GO:0032878 regulation of establishment or maintenance of cell polarity; GO:0035264 multicellular organism growth; GO:0045725 positive regulation of glycogen biosynthetic process; GO:0048009 insulin-like growth factor receptor signaling pathway; GO:0048286 lung alveolus development; GO:0048468 cell development; GO:0048754 branching morphogenesis of a tube; GO:0050650 chondroitin sulfate proteoglycan biosynthetic process; GO:0051897 positive regulation of protein kinase B signaling cascade; GO:0060426 lung vasculature development; GO:0060463 lung lobe morphogenesis; GO:0060509 Type I pneumocyte differentiation; GO:0060510 Type II pneumocyte differentiation; GO:0060527 prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis; GO:0060736 prostate gland growth; GO:0060741 prostate gland stromal morphogenesis; GO:0060766 negative regulation of androgen receptor signaling pathway; GO:0070373 negative regulation of ERK1 and ERK2 cascade
Regulatory: For research purposes only

Applications of IGF-I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.5	2.0ug/ml
Functional Assays	12.0	14.0ug/ml
Western Blotting	0.1	0.2ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

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