Rabbit anti Mouse GM-CSF antibody recognizes mouse granulocyte-macrophage colony-stimulating factor (GM-CSF), a haematopoietic growth factor which exists in both glycosylated and non-glycosylated biologically active forms and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The ability of recombinant GM-CSF to increase haematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to mouse GM-CSF were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant mouse GM-CSF.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of reconstitution.
- Entrez Gene
- GO Terms
embryonic placenta development
granulocyte macrophage colony-stimulating factor receptor binding
growth factor activity
positive regulation of cell proliferation
myeloid dendritic cell differentiation
positive regulation of survival gene product expression
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of GM-CSF antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for GM-CSF antibody
Ding, C. et al. (2005) Diabetes increases expression of ICAM after a brief period of cerebral ischemia.
J Neuroimmunol. 161 (1-2): 61-7.
Mirza, B. et al. (2004) Intracerebral cytokine profiles in adult rats grafted with neural tissue of different immunological disparity.
Brain Res Bull. 63 (2): 105-18.