Fibronectin antibody

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Rabbit anti Mouse Fibronectin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4470-4339 E IF WB 1 ml
Rabbit anti Mouse Fibronectin antibody recognizes fibronectin, a 460-500 kDa glycoprotein involved in tissue organisation and wound repair.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - lyophilised
Reconstitution
Reconstitute in 1 ml of sterile distilled water. For long term storage the addition of a preservative is recommended.
Preparation
Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Ammonium bicarbonate
Preservative Stabilisers
None present
Immunogen
Purified murine fibronectin.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P11276 Related reagents
Entrez Gene
Fn1 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0006953 acute-phase response
GO:0005604 basement membrane
GO:0007044 cell-substrate junction assembly
GO:0007160 cell-matrix adhesion
GO:0008201 heparin binding
GO:0008360 regulation of cell shape
GO:0016324 apical plasma membrane
GO:0016504 peptidase activator activity
GO:0042060 wound healing
Regulatory
For research purposes only

Applications of Fibronectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Western Blotting 1/300 1/400
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Fibronectin antibody

  1. Koya, D. et al. (2000) Amelioration of accelerated diabetic mesangial expansion by treatment with a PKC beta inhibitor in diabetic db/db mice, a rodent model for type 2 diabetes.
    FASEB J. 14 (3): 439-47.
  2. Austin, B.A. et al. (2009) Biologically active fibronectin fragments stimulate release of MCP-1 and catabolic cytokines from murine retinal pigment epithelium.
    Invest Ophthalmol Vis Sci. 50: 2896-902.
  3. Lambeth, L.S. et al. (2015) Transgenic chickens over-expressing aromatase have high estrogen levels but maintain a predominantly male phenotype.
    Endocrinology. : en20151697.
  4. Michalski D et al. (2017) Delayed histochemical alterations within the neurovascular unit due to transient focal cerebral ischemia and experimental treatment with neurotrophic factors.
    PLoS One. 12 (4): e0174996.