Delta-Like Protein 3 antibody
The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression.
Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.
DLL3 is predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis, where it plays a role in somite segmentation, during early development. DLL3 acts as a regulator of Notch 1, as an inhibitor of primary neurogenesis, and studies in mice have linked DLL3-Notch signaling with liver regeneration, and developmental defects. In humans more than 25 mutations in the DLL3 gene are known to be responsible for the autosomal recessive skeletal malformation condition known as spondylocostal dysostosis type 1 (SCDO1), associated with vertebral and rib segmentation defects.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Weak - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to mouse DLL3 was raised by repeated immunisation of rabbits with protein peptide sequence. Purified IgG was prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Keyhole Limpet Hemocyanin (KLH) conjugated peptide sequence CSPEHGYCEEPDE, corresponding to amino acids 222-234 of mouse DLL3.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O88516
- Q9NYJ7
- Entrez Gene
- Dll3
- DLL3
- GO Terms
- GO:0001501 skeletal system development
- GO:0001701 in utero embryonic development
- GO:0001709 cell fate determination
- GO:0001756 somitogenesis
- GO:0016021 integral to membrane
- GO:0005112 Notch binding
- GO:0007219 Notch signaling pathway
- GO:0007386 compartment pattern formation
- GO:0048339 paraxial mesoderm development
- Regulatory
- For research purposes only
Applications of Delta-Like Protein 3 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Western Blotting | 1/100 | 1/500 |
- Western Blotting
- AAM81 detects a prominent band of approximately 60-62kDa in mouse brain tissue, and weakly in human brain tissue.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
Nat Rev Mol Cell Biol. 7 (9): 678-89.
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