CXCL1 antibody

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Rabbit anti Mouse CXCL1:Biotin

Rabbit anti Mouse CXCL1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM40B E WB 50 µg
AAM40 E WB 0.1 mg
Rabbit anti Mouse CXCL1 antibody recognizes mouse CXCL1 also known as GRO1 and KC, a ~7.8 kDa chemokine involved in neutrophil chemotaxis and activation, and expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 1.0ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to mouse CXCL1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant mouse CXCL1
Approx. Protein Concentrations
IgG concentration 50ug/ml after reconstitution.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P12850 Related reagents
Entrez Gene
Cxcl1 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0008083 growth factor activity
Regulatory
For research purposes only

Applications of CXCL1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.15ug/ml 0.30ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM40B may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAM40 as the capture antibody and PMP66 as the standard.
ELISA
AAM40 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with AAM40B as the detection antibody and PMP66 as the standard.
Western Blotting
AAM40B may be used under either reducing or non-reducing western blotting conditions with PMP66 as the positive control.
Western Blotting
AAM40 may be used under either reducing or non-reducing western blotting conditions with PMP66 as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Mouse CXCL1 AAM40 0.1 mg E WB
Rabbit anti Mouse CXCL1:Biotin AAM40B 50 µg E WB
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Positive Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Mouse CXCL1 PMP66 20 µg E FN WB
Recombinant Mouse CXCL1 PMP66 20 µg E FN WB

Product Specific References

References for CXCL1 antibody

  1. Liu, H. et al. (2017) NLRP3 inflammasome induced liver graft injury through activation of telomere-independent RAP1/KC axis.
    J Pathol. 242 (3): 284-96.