CXCL1 antibody

Rabbit anti Mouse CXCL1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CXCL1

Product Code Applications Pack Size List Price Your Price Qty
AAM40
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti Mouse CXCL1 antibody recognizes mouse CXCL1 also known as GRO1 and KC, a ~7.8 kDa chemokine involved in neutrophil chemotaxis and activation, and expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to mouse CXCL1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM40 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with AAM40B as the detection antibody and PMP66 as the standard.
Western Blotting
AAM40 may be used under either reducing or non-reducing western blotting conditions with PMP66 as the positive control.

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References for CXCL1 antibody

  1. Liu, H. et al. (2017) NLRP3 inflammasome induced liver graft injury through activation of telomere-independent RAP1/KC axis.
    J Pathol. 242 (3): 284-96.

Synonyms
GRO1
RRID
AB_2087692
UniProt
P12850
Entrez Gene
Cxcl1
GO Terms
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0008083 growth factor activity
View more products with CXCL1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products