CXCL1 antibody
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Rabbit anti Mouse CXCL1 antibody recognizes mouse CXCL1 also known as GRO1 and KC, a ~7.8 kDa chemokine involved in neutrophil chemotaxis and activation, and expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Antiserum Preparation
- Antisera to mouse CXCL1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant mouse CXCL1
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
- Shelf Life
- 3 months from date of reconstitution.
Please see label for expiry date.
More Information
- UniProt
- P12850 Related reagents
- Entrez Gene
- Cxcl1 Related reagents
- GO Terms
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008009 chemokine activity
- GO:0008083 growth factor activity
- Regulatory
- For research purposes only
Applications of CXCL1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.15ug/ml | 0.30ug/ml | |
| Western Blotting | 0.1ug/ml | 0.2ug/ml | |
| ELISA | 0.5 | 2.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- ELISA
- AAM40B may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAM40 as the capture antibody and PMP66 as the standard.
- ELISA
- AAM40 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with AAM40B as the detection antibody and PMP66 as the standard.
- Western Blotting
- AAM40B may be used under either reducing or non-reducing western blotting conditions with PMP66 as the positive control.
- Western Blotting
- AAM40 may be used under either reducing or non-reducing western blotting conditions with PMP66 as the positive control.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Mouse CXCL1 | AAM40 | 0.1 mg | E WB | ||
| Rabbit anti Mouse CXCL1:Biotin | AAM40B | 50 µg | E WB | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Positive Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Mouse CXCL1 | PMP66 | 20 µg | E FN WB | ||
| Recombinant Mouse CXCL1 | PMP66 | 20 µg | E FN WB |
Product Specific References
References for CXCL1 antibody
-
Liu, H. et al. (2017) NLRP3 inflammasome induced liver graft injury through activation of telomere-independent RAP1/KC axis.
J Pathol. 242 (3): 284-96.
