CD95 / FAS antibody
Rabbit anti CD95 / FAS antibody recognizes the CD95 antigen, also known as apoptosis-mediating surface antigen Fas and TNFRSF6 (tumor necrosis factor receptor superfamily member 6). Upon ligand binding the Fas receptor forms the death-inducing signaling complex (DISC), which mediates apoptosis.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Expected from Sequence Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
5mM EDTA - Immunogen
- Synthetic peptide corresponding to residues surrounding amino acid 32 of mouse CD95 / FAS
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P25446
- Entrez Gene
- Fas
- GO Terms
- GO:0002377 immunoglobulin production
- GO:0003014 renal system process
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0006924 activation-induced cell death of T cells
- GO:0006925 inflammatory cell apoptosis
- GO:0006927 transformed cell apoptosis
- GO:0008625 induction of apoptosis via death domain receptors
- GO:0009636 response to toxin
- GO:0009897 external side of plasma membrane
- GO:0010467 gene expression
- GO:0019724 B cell mediated immunity
- GO:0051260 protein homooligomerization
- GO:0043066 negative regulation of apoptosis
- GO:0045060 negative thymic T cell selection
- GO:0045619 regulation of lymphocyte differentiation
- GO:0045637 regulation of myeloid cell differentiation
- GO:0048536 spleen development
- GO:0050869 negative regulation of B cell activation
- GO:0051384 response to glucocorticoid stimulus
- GO:0051789 response to protein stimulus
- Regulatory
- For research purposes only
Applications of CD95 / FAS antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 5 ug/ml | ||
| Western Blotting | 0.5 | 4.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti CD95 / Fas antibody recognizes the glycosylated form of CD95 / Fas (approximately 55 kDa), the native form of CD95 / Fas (approximately 35 kDa) and a cleaved CD95 / Fas fragment (approximately 29 kDa) in rat kidney tissue lysate
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
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