CD289 antibody

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Anti Mouse CD289 (N-Terminal) Antibody thumbnail image 1

Anti Mouse CD289 (N-Terminal) Antibody thumbnail image 2

Anti Mouse CD289 (N-Terminal) Antibody gallery image 1 — Staining of paraffin embedded mouse thymus with Goat anti mouse CD289 (**AAM74**).

Anti Mouse CD289 (N-Terminal) Antibody gallery image 2 — Staining of paraffin embedded human spleen with Goat anti mouse CD289 (**AAM74**).

Goat anti Mouse CD289 (N-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AAM74 Datasheet	P WB MSDS	0.1 mg	Log in		Get Quote

Goat anti Mouse CD289 antibody recognises murine Toll-like receptor 9, also known as CD289 or TLR9. CD289 is an 1032 amino acid ~120 kDa endosomal single pass type I transmembrane glycoprotein with a number of leucine rich repeats and a single TIR domain (UniProt: Q9NR96). Goat anti Mouse CD289 antibody recognizes an epitope within the N-terminal (NT) region of CD289, a member of the toll-like receptor family, which triggers MyD88 and TRAF6, resulting in NF-kappaB activation, cytokine secretion and the inflammatory response.

CD289 acts as a receptor for unmethylated cytidine-phosphate-guanosine dinucleotides in bacterial DNA (CpG-DNA), playing a primary role in pathogen recognition and activation of innate immunity and has been shown to play a role in defence against systemic mouse cytomegalovirus infection.

Goat anti Mouse CD289 antibody is reported as suitable for use in immunocytochemistry on acetone fixed cells.

Product Details

Target Species

Mouse

Species Cross-Reactivity

Target Species	Cross Reactivity
Human

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to mouse CD289 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
0.1% Bovine Serum Albumin

Immunogen

Synthetic peptide LSLKYNNLTKVPRQLPPSLEY-C corresponding to amino acids 204-224 within the N-terminal region of mouse CD289.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q9NR96; Q9EQU3
Entrez Gene: Tlr9; TLR9
GO Terms: GO:0005886 plasma membrane; GO:0005515 protein binding; GO:0002237 response to molecule of bacterial origin; GO:0005764 lysosome; GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0005768 endosome; GO:0005789 endoplasmic reticulum membrane; GO:0006954 inflammatory response; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0030277 maintenance of gastrointestinal epithelium; GO:0032009 early phagosome; GO:0032715 negative regulation of interleukin-6 production; GO:0032728 positive regulation of interferon-beta production; GO:0032733 positive regulation of interleukin-10 production; GO:0032735 positive regulation of interleukin-12 production; GO:0032741 positive regulation of interleukin-18 production; GO:0032755 positive regulation of interleukin-6 production; GO:0032760 positive regulation of tumor necrosis factor production; GO:0035197 siRNA binding; GO:0045078 positive regulation of interferon-gamma biosynthetic process; GO:0045087 innate immune response; GO:0045356 positive regulation of interferon-alpha biosynthetic process; GO:0045359 positive regulation of interferon-beta biosynthetic process; GO:0050727 regulation of inflammatory response; GO:0051607 defense response to virus; GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process; GO:0042742 defense response to bacterium; GO:0005576 extracellular region; GO:0005149 interleukin-1 receptor binding; GO:0007252 I-kappaB phosphorylation; GO:0008286 insulin receptor signaling pathway; GO:0008543 fibroblast growth factor receptor signaling pathway; GO:0010008 endosome membrane; GO:0016323 basolateral plasma membrane; GO:0016324 apical plasma membrane; GO:0032088 negative regulation of NF-kappaB transcription factor activity; GO:0032717 negative regulation of interleukin-8 production; GO:0032722 positive regulation of chemokine production; GO:0032757 positive regulation of interleukin-8 production; GO:0034122 negative regulation of toll-like receptor signaling pathway; GO:0034123 positive regulation of toll-like receptor signaling pathway; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043507 positive regulation of JUN kinase activity; GO:0046330 positive regulation of JNK cascade; GO:0050729 positive regulation of inflammatory response; GO:0051092 positive regulation of NF-kappaB transcription factor activity
Regulatory: For research purposes only

Applications of CD289 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin		1:125
Western Blotting		1:500

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Spleen, thymus or tonsil

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

Product Specific References

References for CD289 antibody

Ripoll, V.M. et al. (2010) Macrophages from BALB/c and CBA/Ca mice differ in their cellular responses to Streptococcus pneumoniae.
J Leukoc Biol. 87: 735-41.

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