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CD289 antibody

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Anti Mouse CD289 (N-Terminal) Antibody thumbnail image 1 Anti Mouse CD289 (N-Terminal) Antibody thumbnail image 2
Anti Mouse CD289 (N-Terminal) Antibody gallery image 1
Staining of paraffin embedded mouse thymus with Goat anti mouse CD289 (AAM74).
Anti Mouse CD289 (N-Terminal) Antibody gallery image 2
Staining of paraffin embedded human spleen with Goat anti mouse CD289 (AAM74).

Goat anti Mouse CD289 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAM74
Datasheet
P WB
SDS
0.1 mg loader

Goat anti Mouse CD289 antibody recognises murine Toll-like receptor 9, also known as CD289 or TLR9. CD289 is an 1032 amino acid ~120 kDa endosomal single pass type I transmembrane glycoprotein with a number of leucine rich repeats and a single TIR domain (UniProt: Q9NR96). Goat anti Mouse CD289 antibody recognizes an epitope within the N-terminal (NT) region of CD289, a member of the toll-like receptor family, which triggers MyD88 and TRAF6, resulting in NF-kappaB activation, cytokine secretion and the inflammatory response.

CD289 acts as a receptor for unmethylated cytidine-phosphate-guanosine dinucleotides in bacterial DNA (CpG-DNA), playing a primary role in pathogen recognition and activation of innate immunity and has been shown to play a role in defence against systemic mouse cytomegalovirus infection.

Goat anti Mouse CD289 antibody is reported as suitable for use in immunocytochemistry on acetone fixed cells.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mouse CD289 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Synthetic peptide LSLKYNNLTKVPRQLPPSLEY-C corresponding to amino acids 204-224 within the N-terminal region of mouse CD289.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9NR96
Q9EQU3
Entrez Gene
Tlr9
TLR9
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0002237 response to molecule of bacterial origin
GO:0005764 lysosome
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005768 endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006954 inflammatory response
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0030277 maintenance of gastrointestinal epithelium
GO:0032009 early phagosome
GO:0032715 negative regulation of interleukin-6 production
GO:0032728 positive regulation of interferon-beta production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032741 positive regulation of interleukin-18 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0035197 siRNA binding
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045087 innate immune response
GO:0045356 positive regulation of interferon-alpha biosynthetic process
GO:0045359 positive regulation of interferon-beta biosynthetic process
GO:0050727 regulation of inflammatory response
GO:0051607 defense response to virus
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
GO:0042742 defense response to bacterium
GO:0005576 extracellular region
GO:0005149 interleukin-1 receptor binding
GO:0007252 I-kappaB phosphorylation
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0010008 endosome membrane
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032717 negative regulation of interleukin-8 production
GO:0032722 positive regulation of chemokine production
GO:0032757 positive regulation of interleukin-8 production
GO:0034122 negative regulation of toll-like receptor signaling pathway
GO:0034123 positive regulation of toll-like receptor signaling pathway
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043507 positive regulation of JUN kinase activity
GO:0046330 positive regulation of JNK cascade
GO:0050729 positive regulation of inflammatory response
GO:0051092 positive regulation of NF-kappaB transcription factor activity
Regulatory
For research purposes only

Applications of CD289 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1:125
Western Blotting 1:500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Spleen, thymus or tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Product Specific References

References for CD289 antibody

  1. Ripoll, V.M. et al. (2010) Macrophages from BALB/c and CBA/Ca mice differ in their cellular responses to Streptococcus pneumoniae.
    J Leukoc Biol. 87: 735-41.

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