Goat anti Mouse CD289 antibody
recognises murine Toll-like receptor 9, also known as CD289 or TLR9. CD289 is an 1032 amino acid ~120 kDa endosomal single pass type I transmembrane glycoprotein with a number of leucine rich repeats
and a single TIR
domain (UniProt: Q9NR96
). Goat anti Mouse CD289 antibody recognizes an epitope within the N-terminal (NT) region of CD289, a member of the toll-like receptor family, which triggers MyD88 and TRAF6, resulting in NF-kappaB activation, cytokine secretion and the inflammatory response.
CD289 acts as a receptor for unmethylated cytidine-phosphate-guanosine dinucleotides in bacterial DNA (CpG-DNA), playing a primary role in pathogen recognition and activation of innate immunity and has been shown to play a role in defence against systemic mouse cytomegalovirus infection.
Goat anti Mouse CD289 antibody is reported as suitable for use in immunocytochemistry on acetone fixed cells.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to mouse CD289 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
- Synthetic peptide LSLKYNNLTKVPRQLPPSLEY-C corresponding to amino acids 204-224 within the N-terminal region of mouse CD289.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
response to molecule of bacterial origin
integral to membrane
transmembrane receptor activity
endoplasmic reticulum membrane
positive regulation of gene-specific transcription from RNA polymerase II promoter
maintenance of gastrointestinal epithelium
negative regulation of interleukin-6 production
positive regulation of interferon-beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-18 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of interferon-gamma biosynthetic process
innate immune response
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
regulation of inflammatory response
defense response to virus
positive regulation of nitric-oxide synthase biosynthetic process
defense response to bacterium
interleukin-1 receptor binding
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
basolateral plasma membrane
apical plasma membrane
negative regulation of NF-kappaB transcription factor activity
negative regulation of interleukin-8 production
positive regulation of chemokine production
positive regulation of interleukin-8 production
negative regulation of toll-like receptor signaling pathway
positive regulation of toll-like receptor signaling pathway
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB cascade
positive regulation of JUN kinase activity
positive regulation of JNK cascade
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD289 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Spleen, thymus or tonsil
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for CD289 antibody
Ripoll, V.M. et al. (2010) Macrophages from BALB/c and CBA/Ca mice differ in their cellular responses to Streptococcus pneumoniae.
J Leukoc Biol. 87: 735-41.
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