Caspase-12 antibody

Rabbit anti Caspase-12 (aa2-17)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Caspase-12
Region
(aa2-17)

Product Code Applications Pack Size List Price Your Price Qty
AHP626
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti caspase-12 (aa12-17) polyclonal antibody recognizes an epitope within the N-terminal region of murine caspase 12. Caspase 12 is a member of the interleukin-1 Beta converting enzyme (ICE) subfamily of caspases. Caspase 12 is ubiquitously expressed in murine tissue and is localised to the endoplasmic reticulum.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to mouse caspase 12 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 2-17 of murine caspase 12.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10ug/ml
Western Blotting 0.5ug/ml 1ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Heart tissue.

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Further Reading

  1. VanDeCraen, M. et al. (1997) Characterization of seven murine caspase family members.
    FEBS Lett. 403 (1): 61-9.

RRID
AB_323642
UniProt
O08736
Entrez Gene
Casp12
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005783 endoplasmic reticulum
GO:0006926 virus-infected cell apoptosis
GO:0030968 endoplasmic reticulum unfolded protein response
GO:0070059 apoptosis in response to endoplasmic reticulum stress
View more products with CASPASE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products