CAD antibody

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Rabbit anti Mouse CAD (aa205-222)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP486 P* WB 0.1 mg
Rabbit anti Murine CAD antibody recognizes murine CAD (Caspase Activated Deoxyribonuclease), the murine homologue of human DFF-40.

CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA.

Rabbit anti Murine CAD antibody recognizes a band of ~40 kDa in western blotting.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to murine CAD were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 205-222 of mouse CAD.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O54788 Related reagents
Entrez Gene
Dffb Related reagents
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0004516 nicotinate phosphoribosyltransferase activity
GO:0005634 nucleus
GO:0005737 cytoplasm
Regulatory
For research purposes only

Applications of CAD antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1/500 1/1000
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Enari, M. et al. (1998) A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.
    Nature. 391 (6662): 43-50.
  2. Sakahira, H. et al. (1998) Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis.
    Nature. 391 (6662): 96-9.
  3. Liu, X. et al. (1997) DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.
    Cell. 89 (2): 175-84.