CAD antibody
Rabbit anti Murine CAD antibody recognizes murine CAD (Caspase Activated Deoxyribonuclease), the murine homologue of human DFF-40.
CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA.
Rabbit anti Murine CAD antibody recognizes a band of ~40 kDa in western blotting.
CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA.
Rabbit anti Murine CAD antibody recognizes a band of ~40 kDa in western blotting.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to murine CAD were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Synthetic peptide corresponding to amino acids 205-222 of mouse CAD.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O54788
- Entrez Gene
- Dffb
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0004516 nicotinate phosphoribosyltransferase activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- Regulatory
- For research purposes only
Applications of CAD antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 1/500 | 1/1000 |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Enari, M. et al. (1998) A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.
Nature. 391 (6662): 43-50. -
Sakahira, H. et al. (1998) Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis.
Nature. 391 (6662): 96-9. -
Liu, X. et al. (1997) DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.
Cell. 89 (2): 175-84.
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