CAD antibody

Rabbit anti Mouse CAD (aa205-222)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CAD
Region
(aa205-222)

Product Code Applications Pack Size List Price Your Price Qty
AHP486
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Murine CAD antibody recognizes murine CAD (Caspase Activated Deoxyribonuclease), the murine homologue of human DFF-40.

CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA.

Rabbit anti Murine CAD antibody recognizes a band of ~40 kDa in western blotting.

Target Species
Mouse
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to murine CAD were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 205-222 of mouse CAD.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1/500 1/1000
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
List Price Your Price
loader
Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

Further Reading

  1. Enari, M. et al. (1998) A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.
    Nature. 391 (6662): 43-50.
  2. Sakahira, H. et al. (1998) Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis.
    Nature. 391 (6662): 96-9.
  3. Liu, X. et al. (1997) DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.
    Cell. 89 (2): 175-84.

RRID
AB_322892
UniProt
O54788
Entrez Gene
Dffb
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0004516 nicotinate phosphoribosyltransferase activity
GO:0005634 nucleus
GO:0005737 cytoplasm

AHP486

170130

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CAD specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products