mCherry antibody

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Rabbit anti mCherry

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2326
Datasheet
WB
MSDS
50 µg loader

Rabbit anti mCherry antibody recognizes the red fluorescent mCherry protein, a monomeric engineered derivate of a fluorescent protein isolated from members of the Discosoma (mushroom coral) family. mCherry is a second generation fluorescent protein and by far the most popular red emitting fluorescent protein due to its high photostability and size (the other photostable red fluorescent protein tdTomato due to its tandem dimer nature is significantly larger than the 29 kDa mCherry protein). mCherry is optimally excited at a wavelength of 587nm and emits light at 610nm.

Fluorescent proteins are used as tags in the research environment and are fused to the N- or C-terminus of proteins of interest. They are tools enabling the visualization of proteins, protein-protein interactions and are used in cell-sorting and FRET, FRAP and FLIM imaging experiments.

Product Details

Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.03% ProclinTM300
0.5mM EDTA
Immunogen
E. coli expressed recombinant mCherry
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of mCherry antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti mCherry antibody recognizes a band of approximately 28.8 kDa on recombinant mCherry

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Green Fluorescent Protein AHP2984 WB 50 µg loader
Rabbit anti Blue Fluorescent Protein AHP2985 WB 50 µg loader
Rabbit anti Cyan Fluorescent Protein AHP2986 WB 50 µg loader
Rabbit anti Red Fluorescent Protein AHP2987 WB 50 µg loader