XPA antibody

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Rabbit anti Human XPA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP452
Datasheet
P* WB
MSDS
0.1 ml loader

Rabbit anti Human XPA antibody recognizes the human DNA repair associated protein designated XPA (Xeroderma pigmentosum A).XPA is a zinc finger protein of ~31 kDa, although it demonstrates an electophoretic mobility of a~40-42 kDa doublet. XPA is involved in the nucleotide excision repair process.

Rabbit anti Human XPA antibody recognises both normal and recombinant XPA, and shows no cross reactivity with other XP proteins.

Product Details

Target Species
Human
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human XPA were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant human XPA.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P23025 Related reagents
Entrez Gene
XPA Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0003684 damaged DNA binding
GO:0046872 metal ion binding
GO:0005654 nucleoplasm
GO:0019904 protein domain specific binding
GO:0042803 protein homodimerization activity
Regulatory
For research purposes only

Applications of XPA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/50
Western Blotting 1/5000
  1. 1This product requires heat mediated treatment of paraffin sections. Bio-Rad recommend the use of antigen unmasking fluid for this purpose. A two hour incubation with AHP452 is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Western Blotting

Product Specific References

References for XPA antibody

  1. Jones, C.J. & Wood, R.D. (1993) Preferential binding of the xeroderma pigmentosum group A complementing protein to damaged DNA.
    Biochemistry. 32 (45): 12096-104.
  2. Li, R.Y. et al. (1998) Interactions of the transcription/DNA repair factor TFIIH and XP repair proteins with DNA lesions in a cell-free repair assay.
    J Mol Biol. 281 (2): 211-8.
  3. Araújo, S.J. et al. (2001) Strong functional interactions of TFIIH with XPC and XPG in human DNA nucleotide excision repair, without a preassembled repairosome.
    Mol Cell Biol. 21 (7): 2281-91.