Rabbit anti Human XPA antibody recognizes the human DNA repair associated protein designated XPA (Xeroderma pigmentosum A).XPA is a zinc finger protein of ~31 kDa, although it demonstrates an electophoretic mobility of a~40-42 kDa doublet. XPA is involved in the nucleotide excision repair process.
Rabbit anti Human XPA antibody recognises both normal and recombinant XPA, and shows no cross reactivity with other XP proteins.
- Target Species
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to human XPA were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- Recombinant human XPA.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
nucleotide-excision repair, DNA damage removal
damaged DNA binding
metal ion binding
protein domain specific binding
protein homodimerization activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of XPA antibody
|Immunohistology - Paraffin 1
- 1This product requires heat mediated treatment of paraffin sections. Bio-Rad recommend the use of antigen unmasking fluid for this purpose. A two hour incubation with AHP452 is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for XPA antibody
Jones, C.J. & Wood, R.D. (1993) Preferential binding of the xeroderma pigmentosum group A complementing protein to damaged DNA.
Biochemistry. 32 (45): 12096-104.
Li, R.Y. et al. (1998) Interactions of the transcription/DNA repair factor TFIIH and XP repair proteins with DNA lesions in a cell-free repair assay.
J Mol Biol. 281 (2): 211-8.
Araújo, S.J. et al. (2001) Strong functional interactions of TFIIH with XPC and XPG in human DNA nucleotide excision repair, without a preassembled repairosome.
Mol Cell Biol. 21 (7): 2281-91.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra