xCT antibody

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Rabbit anti Human xCT (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1222
Datasheet
P*
SDS
50 µg loader

Rabbit anti human xCT (N-terminal) antibody detects an epitope within the N-terminal (NT) of the cysteine/glutamate transporter xCT, which forms a complex with the 4F2 heavy chain (CD98), via a disulfide bond. This heterodimer forms a Na+ independent amino acid transport system which leads to the uptake of extracellular L-cystine in conjunction to L-glutamate efflux. This system is thought to play an important role in the protection of neurons and activated macrophages against oxidative stress.

xCT is the receptor which mediates Kaposi's sarcoma herpesvirus (KSHV) cell fusion.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human xCT were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Synthetic peptide: N-terminal region of human xCT.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9UPY5
Entrez Gene
SLC7A11
GO Terms
GO:0005886 plasma membrane
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0009636 response to toxin
GO:0015327 cystine:glutamate antiporter activity
GO:0034641 cellular nitrogen compound metabolic process
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of xCT antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 20 30ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Neurons or astrocytes

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

References for xCT antibody

  1. Kaleeba, J.A. & Berger, E.A. (2006) Kaposi's sarcoma-associated herpesvirus fusion-entry receptor: cystine transporter xCT.
    Science. 311 (5769): 1921-4.
  2. Wang, H. et al. (2003) Expression of the activity of cystine/glutamate exchange transporter, system x(c)(-), by xCT and rBAT.
    Biochem Biophys Res Commun. 305 (3): 611-8.
  3. Jiménez-vidal, M. et al. (2004) Thiol modification of cysteine 327 in the eighth transmembrane domain of the light subunit xCT of the heteromeric cystine/glutamate antiporter suggests close proximity to the substrate binding site/permeation pathway.
    J Biol Chem. 279 (12): 11214-21.