Rabbit anti human xCT (N-terminal) antibody detects an epitope within the N-terminal (NT) of the cysteine/glutamate transporter xCT, which forms a complex with the 4F2 heavy chain (CD98), via a disulfide bond. This heterodimer forms a Na+ independent amino acid transport system which leads to the uptake of extracellular L-cystine in conjunction to L-glutamate efflux. This system is thought to play an important role in the protection of neurons and activated macrophages against oxidative stress.
xCT is the receptor which mediates Kaposi's sarcoma herpesvirus (KSHV) cell fusion.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human xCT were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Synthetic peptide: N-terminal region of human xCT.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
response to toxin
cystine:glutamate antiporter activity
cellular nitrogen compound metabolic process
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of xCT antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Neurons or astrocytes
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for xCT antibody
Kaleeba, J.A. & Berger, E.A. (2006) Kaposi's sarcoma-associated herpesvirus fusion-entry receptor: cystine transporter xCT.
Science. 311 (5769): 1921-4.
Wang, H. et al. (2003) Expression of the activity of cystine/glutamate exchange transporter, system x(c)(-), by xCT and rBAT.
Biochem Biophys Res Commun. 305 (3): 611-8.
Jiménez-vidal, M. et al. (2004) Thiol modification of cysteine 327 in the eighth transmembrane domain of the light subunit xCT of the heteromeric cystine/glutamate antiporter suggests close proximity to the substrate binding site/permeation pathway.
J Biol Chem. 279 (12): 11214-21.
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