WRN antibody

100% Secure


Rabbit anti WRN

WRN antibody recognizes Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, RecQ3, exonuclease WRN or RecQ protein-like 2. The antibody has been extensively validated for western blotting using whole cell lysates.

WRN Antibody with Control Lysate

WRN antibody recognizes Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, RecQ3, exonuclease WRN or RecQ protein-like 2. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00006 WB 100 µl
VPA00006KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human WRN antibody recognizes human Werner syndrome ATP-dependent helicase, also known as exonuclease WRN or DNA helicase, RecQ-like type 3. WRN is a 1432 amino acid member of the RecQ helicase subfamily containing single HRDC, helicase C-terminal, helicase ATP-binding and 3'-5' exonuclease domains and is implicated in DNA double-strand break repairs, through interaction with Ku70/80 (Li et al. 2004).

Defects in the WRN gene are responsible for the rare autosomal recessive premature ageing disease known as Werner syndrome, characterized by age-related disorders including atherosclerosis, osteoporosis, non-insulin-dependent diabetes mellitus, ocular cataracts and cancer (Huang et al. 2006).

Rabbit anti Human WRN antibody recognizes human WRN as a single ~200 kDa band in HeLa cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti WRN antibody detects a band of approximately 200 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS-citrate/Phosphate buffered saline
Buffer Solution
TRIS-citrate/Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an epitope within amino acid residues 1400-1432 of human WRN
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14191 Related reagents
Entrez Gene
WRN Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0000403 Y-form DNA binding
GO:0000405 bubble DNA binding
GO:0000723 telomere maintenance
GO:0000731 DNA synthesis involved in DNA repair
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005813 centrosome
GO:0006284 base-excision repair
GO:0006310 DNA recombination
GO:0006979 response to oxidative stress
GO:0008408 3'-5' exonuclease activity
GO:0009378 four-way junction helicase activity
GO:0010225 response to UV-C
GO:0010259 multicellular organismal aging
GO:0042981 regulation of apoptosis
GO:0030145 manganese ion binding
GO:0031297 replication fork processing
GO:0032403 protein complex binding
GO:0042803 protein homodimerization activity
GO:0043140 ATP-dependent 3'-5' DNA helicase activity
GO:0051345 positive regulation of hydrolase activity
GO:0051880 G-quadruplex DNA binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of WRN antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted