WRN antibody

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Anti WRN Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with WRN antibody (**VPA00006**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to WRN (molecular weight 200 kDa).

Rabbit anti WRN

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: WRN

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00006 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human WRN antibody recognizes human Werner syndrome ATP-dependent helicase, also known as exonuclease WRN or DNA helicase, RecQ-like type 3. WRN is a 1432 amino acid member of the RecQ helicase subfamily containing single HRDC, helicase C-terminal, helicase ATP-binding and 3'-5' exonuclease domains and is implicated in DNA double-strand break repairs, through interaction with Ku70/80 (Li et al. 2004).

Defects in the WRN gene are responsible for the rare autosomal recessive premature ageing disease known as Werner syndrome, characterized by age-related disorders including atherosclerosis, osteoporosis, non-insulin-dependent diabetes mellitus, ocular cataracts and cancer (Huang et al. 2006).

Rabbit anti Human WRN antibody recognizes human WRN as a single ~200 kDa band in HeLa cell line lysates by western blotting under reducing conditions.

Target Species: Human
Western Blotting: Anti WRN antibody detects a band of approximately 200 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS-citrate/Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Synthetic peptide corresponding to an epitope within amino acid residues 1400-1432 of human WRN
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

Synonyms: Werner Syndrome Helicase
UniProt: Q14191
Entrez Gene: WRN
GO Terms: GO:0000287 magnesium ion binding; GO:0000403 Y-form DNA binding; GO:0000405 bubble DNA binding; GO:0000723 telomere maintenance; GO:0000731 DNA synthesis involved in DNA repair; GO:0005524 ATP binding; GO:0005654 nucleoplasm; GO:0005730 nucleolus; GO:0005813 centrosome; View More GO Terms; GO:0006284 base-excision repair; GO:0006310 DNA recombination; GO:0006979 response to oxidative stress; GO:0008408 3'-5' exonuclease activity; GO:0009378 four-way junction helicase activity; GO:0010225 response to UV-C; GO:0010259 multicellular organismal aging; GO:0042981 regulation of apoptosis; GO:0030145 manganese ion binding; GO:0031297 replication fork processing; GO:0032403 protein complex binding; GO:0042803 protein homodimerization activity; GO:0043140 ATP-dependent 3'-5' DNA helicase activity; GO:0051345 positive regulation of hydrolase activity; GO:0051880 G-quadruplex DNA binding