WRN antibody
Rabbit anti Human WRN antibody recognizes human Werner syndrome ATP-dependent helicase, also known as exonuclease WRN or DNA helicase, RecQ-like type 3. WRN is a 1432 amino acid member of the RecQ helicase subfamily containing single HRDC, helicase C-terminal, helicase ATP-binding and 3'-5' exonuclease domains and is implicated in DNA double-strand break repairs, through interaction with Ku70/80 (Li et al. 2004).
Defects in the WRN gene are responsible for the rare autosomal recessive premature ageing disease known as Werner syndrome, characterized by age-related disorders including atherosclerosis, osteoporosis, non-insulin-dependent diabetes mellitus, ocular cataracts and cancer (Huang et al. 2006).
Rabbit anti Human WRN antibody recognizes human WRN as a single ~200 kDa band in HeLa cell line lysates by western blotting under reducing conditions.
Defects in the WRN gene are responsible for the rare autosomal recessive premature ageing disease known as Werner syndrome, characterized by age-related disorders including atherosclerosis, osteoporosis, non-insulin-dependent diabetes mellitus, ocular cataracts and cancer (Huang et al. 2006).
Rabbit anti Human WRN antibody recognizes human WRN as a single ~200 kDa band in HeLa cell line lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti WRN antibody detects a band of approximately 200 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS-citrate/Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to an epitope within amino acid residues 1400-1432 of human WRN
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q14191
- Entrez Gene
- WRN
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0000403 Y-form DNA binding
- GO:0000405 bubble DNA binding
- GO:0000723 telomere maintenance
- GO:0000731 DNA synthesis involved in DNA repair
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0005813 centrosome
- GO:0006284 base-excision repair
- GO:0006310 DNA recombination
- GO:0006979 response to oxidative stress
- GO:0008408 3'-5' exonuclease activity
- GO:0009378 four-way junction helicase activity
- GO:0010225 response to UV-C
- GO:0010259 multicellular organismal aging
- GO:0042981 regulation of apoptosis
- GO:0030145 manganese ion binding
- GO:0031297 replication fork processing
- GO:0032403 protein complex binding
- GO:0042803 protein homodimerization activity
- GO:0043140 ATP-dependent 3'-5' DNA helicase activity
- GO:0051345 positive regulation of hydrolase activity
- GO:0051880 G-quadruplex DNA binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of WRN antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
