WNT2B antibody

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Rabbit anti WNT2B

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1136 P* 50 µg
Rabbit anti Human Wnt2b antibody detects an epitope within the internal region of human Wnt2b, also known as Wnt13. Wnt2b is a signalling factor which acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is thought to play a key role during mammalian early embryogenesis, and may also function as a stem cell factor for neural and retinal progenitor cells.

The Wnt2b gene encodes two alternative transcript variants; Wnt2B splicing variant 1 encodes secreted-type glycoprotein (Wnt2B isoform 1), while Wnt2B splicing variant 2 encodes a transmembrane-type glycoprotein (Wnt2B isoform 2).

Wnt2b has been implicated in gastric cancer, esophageal cancer and skin basal cell carcinoma (Katoh 2005).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Wnt2b were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an internal domain of human Wnt2b.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q93097 Related reagents
Entrez Gene
WNT2B Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0002062 chondrocyte differentiation
GO:0002088 lens development in camera-type eye
GO:0004871 signal transducer activity
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
GO:0005110 frizzled-2 binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0008584 male gonad development
GO:0009950 dorsal/ventral axis specification
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0021871 forebrain regionalization
GO:0030182 neuron differentiation
GO:0060492 lung induction
GO:0061072 iris morphogenesis
GO:0061303 cornea development in camera-type eye
GO:0071425 hemopoietic stem cell proliferation
GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of WNT2B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain, cerebellum.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

References for WNT2B antibody

  1. Katoh, M. (2005) WNT2B: comparative integromics and clinical applications (Review).
    Int J Mol Med. 16 (6): 1103-8.