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WNT2B antibody

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Anti WNT2B Antibody gallery image 1
Staining of formalin fixed, paraffin embedded human cerebellum with Rabbit anti Human WNT28 (AHP1136).

Rabbit anti WNT2B

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1136
Datasheet
P*
SDS
50 µg loader

Rabbit anti Human Wnt2b antibody detects an epitope within the internal region of human Wnt2b, also known as Wnt13. Wnt2b is a signalling factor which acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is thought to play a key role during mammalian early embryogenesis, and may also function as a stem cell factor for neural and retinal progenitor cells.

The Wnt2b gene encodes two alternative transcript variants; Wnt2B splicing variant 1 encodes secreted-type glycoprotein (Wnt2B isoform 1), while Wnt2B splicing variant 2 encodes a transmembrane-type glycoprotein (Wnt2B isoform 2).

Wnt2b has been implicated in gastric cancer, esophageal cancer and skin basal cell carcinoma (Katoh 2005).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Wnt2b were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an internal domain of human Wnt2b.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q93097
Entrez Gene
WNT2B
GO Terms
GO:0005886 plasma membrane
GO:0002062 chondrocyte differentiation
GO:0002088 lens development in camera-type eye
GO:0004871 signal transducer activity
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
GO:0005110 frizzled-2 binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0008584 male gonad development
GO:0009950 dorsal/ventral axis specification
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0021871 forebrain regionalization
GO:0030182 neuron differentiation
GO:0060492 lung induction
GO:0061072 iris morphogenesis
GO:0061303 cornea development in camera-type eye
GO:0071425 hemopoietic stem cell proliferation
GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of WNT2B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain, cerebellum.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

References for WNT2B antibody

  1. Katoh, M. (2005) WNT2B: comparative integromics and clinical applications (Review).
    Int J Mol Med. 16 (6): 1103-8.
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