WNT2B antibody
Rabbit anti Human Wnt2b antibody detects an epitope within the internal region of human Wnt2b, also known as Wnt13. Wnt2b is a signalling factor which acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is thought to play a key role during mammalian early embryogenesis, and may also function as a stem cell factor for neural and retinal progenitor cells.
The Wnt2b gene encodes two alternative transcript variants; Wnt2B splicing variant 1 encodes secreted-type glycoprotein (Wnt2B isoform 1), while Wnt2B splicing variant 2 encodes a transmembrane-type glycoprotein (Wnt2B isoform 2).
Wnt2b has been implicated in gastric cancer, esophageal cancer and skin basal cell carcinoma (Katoh 2005).
The Wnt2b gene encodes two alternative transcript variants; Wnt2B splicing variant 1 encodes secreted-type glycoprotein (Wnt2B isoform 1), while Wnt2B splicing variant 2 encodes a transmembrane-type glycoprotein (Wnt2B isoform 2).
Wnt2b has been implicated in gastric cancer, esophageal cancer and skin basal cell carcinoma (Katoh 2005).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Expected from Sequence Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Wnt2b were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to an internal domain of human Wnt2b.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q93097 Related reagents
- Entrez Gene
- WNT2B Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0002062 chondrocyte differentiation
- GO:0002088 lens development in camera-type eye
- GO:0004871 signal transducer activity
- GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway
- GO:0005110 frizzled-2 binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0005615 extracellular space
- GO:0008584 male gonad development
- GO:0009950 dorsal/ventral axis specification
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0021871 forebrain regionalization
- GO:0030182 neuron differentiation
- GO:0060492 lung induction
- GO:0061072 iris morphogenesis
- GO:0061303 cornea development in camera-type eye
- GO:0071425 hemopoietic stem cell proliferation
- GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
- GO:0090263 positive regulation of canonical Wnt receptor signaling pathway
- Regulatory
- For research purposes only
Applications of WNT2B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 5 | 10ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Brain, cerebellum.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| Histar Detection System | STAR3000A | 50 Tests | C P | ||
| Histar Detection System | STAR3000B | 150 Tests | C P | ||
| Histar Detection System | STAR3000C | 500 Tests | C P |
Product Specific References
References for WNT2B antibody
-
Katoh, M. (2005) WNT2B: comparative integromics and clinical applications (Review).
Int J Mol Med. 16 (6): 1103-8.
