Wilms Tumour 1 antibody

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Rabbit anti Human Wilms Tumor 1

Product Type
Polyclonal Antibody
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1943 IP P* WB 50 µg
Rabbit anti Human Wilms Tumor 1 antibody recognises Wilms Tumor 1, also known asWT1 or WT33. WT1 is a 449 amino acid transcription factor that plays an important role in cellular development, in particular the development of the urogenital system, additionally WT1 functions in both the role of a tumor suppressor and in an oncogenic manner in tumor formation.

Defects in the the WT1 gene result in a range of disease states including Frasier syndrome (FS) characterised by progressive nephropathy, male pseudohermaphroditism with no Wils tumor (Kohsaka et al. 1999), Wilms Tumor 1 (WT1) an embryonal renal malignancy (Little et al. 1992) and Denys-Drash syndrome (DDS) and autosomal dominant nephropathy with genital abnormalities with or without Wils tumor (Baird et al. 1992)

WT1 is expressed in the Kidney and a subset of haematopietic cells. Multiple isoforms of WT1 have so far been recognised. Rabbit anti Human Wilms Tumor 1 antibody is expected to recognize the canonical isoform 1 and other isoforms with a normally presented C-terminal region.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human Wilms Tumor 1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
A synthetic peptide corresponding to amino acid sequence from the C-terminus of human WT1.
Approx. Protein Concentrations
IgG concentration 0.2mg/ml

Storage Information

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

P19544 Related reagents
Entrez Gene
WT1 Related reagents
For research purposes only

Applications of Wilms Tumour 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10ug/ml
Western Blotting
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P