WEE1 antibody

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Rabbit anti WEE1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2358 WB 50 µg
Rabbit anti Wee1 antibody recognizes Wee 1 (Wee1-like protein kinase) also known as Wee1A kinase. The Wee1 tyrosine kinase is an important regulator of the G2 to M transition of the cell cycle via phosphorylating the cdc2/cyclinB kinase. Cdc2 phosphorylated on tyrosine residue 15 by Wee1 kinase and on threonine residue 14 by Myt1 kinase is inactive. Cdc2 becomes active upon dephosphorylation by the cdc25 phosphatase.
Wee1 activity is regulated via phosphorylation on Serine 53 and Serine 123 residues. These modifications trigger Wee1 ubiquitination by the SCFβ-TrCP1/2 E3 ligase complex and subsequent degradation by the 26S proteasome.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
Synthetic peptide mapping to the C-terminus of human WEE1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P47810 Related reagents
Entrez Gene
Wee1 Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0004674 protein serine/threonine kinase activity
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006468 protein phosphorylation
GO:0007067 mitosis
GO:0051301 cell division
Regulatory
For research purposes only

Applications of WEE1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti WEE1 antibody recognizes a band of approximately 72 kDa in mouse small intestine tissue lysate

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB