Rabbit anti Wee1 antibody recognizes Wee 1 (Wee1-like protein kinase) also known as Wee1A kinase. The Wee1 tyrosine kinase is an important regulator of the G2 to M transition of the cell cycle via phosphorylating the cdc2/cyclinB kinase. Cdc2 phosphorylated on tyrosine residue 15 by Wee1 kinase and on threonine residue 14 by Myt1 kinase is inactive. Cdc2 becomes active upon dephosphorylation by the cdc25 phosphatase.
Wee1 activity is regulated via phosphorylation on Serine 53 and Serine 123 residues. These modifications trigger Wee1 ubiquitination by the SCFβ-TrCP1/2 E3 ligase complex and subsequent degradation by the 26S proteasome.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
- Synthetic peptide mapping to the C-terminus of human WEE1
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
magnesium ion binding
protein serine/threonine kinase activity
non-membrane spanning protein tyrosine kinase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of WEE1 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti WEE1 antibody recognizes a band of approximately 72 kDa in mouse small intestine tissue lysate
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Secondary Antibodies Available