VPS34 antibody

Rabbit anti VPS34

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
VPS34

Product Code Applications Pack Size List Price Your Price Qty
AHP2224
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SDS Safety Datasheet SDS
IF WB 0.1 mg loader
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Rabbit anti VPS34 antibody recognises Phosphatidylinositol 3-kinase catalytic subunit type 3, also known as VPS34 (vacuolar protein sorting 34), PI3-kinase type 3 or PIK3C3. VPS34 is a ubiquitously expressed class III PI3K (phosphoinositide 3-kinase), and member of the PI3/PI4-kinase family, which utilizes phosphatidylinositol (PtdIn) as a substrate, and functions as the catalytic subunit of the PI3K complex.

VPS34 plays a role in the transport of lysosomal enzyme precursors to lysosomes, and in endocytosis and vesicular trafficking, and has been strongly implicated as a regulator of autophagy, forming a multi-protein complex with the proautophagic tumor suppressors Beclin1/Atg6, Bif-1, and UVRAG, in the initiation of autophagosome formation. Distinct complexes of VPS34 regulates endocytic processes vital for late-stage autophagosome-lysosome fusion.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human VSP34 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 22-34 of human VPS34, The corresponding sequence is identical in mouse and rat.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/100
Western Blotting 2.0 4.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP224 detects a band of approximately 105kDa in HEK-293T cell lysates.

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Synonyms
PI3-KINASE Type 3
RRID
AB_10845944
UniProt
Q8NEB9
O88763
Q6PF93
Entrez Gene
Pik3c3
Pik3c3
PIK3C3
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0005942 phosphatidylinositol 3-kinase complex
GO:0051605 protein maturation by peptide bond cleavage
GO:0007032 endosome organization
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0043201 response to leucine
GO:0050708 regulation of protein secretion
GO:0004672 protein kinase activity
GO:0006468 protein phosphorylation
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0045087 innate immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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