VPS26A antibody

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Anti VPS26A Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti VPS26A Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti VPS26A Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with VPS26A antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to VPS26A (molecular weight 38 kDa)

Anti VPS26A Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Immunoprecipitation of VPS26A from HEK293 whole cell lysate. The Input lane is 15% input; the VPS26A-IP lane is the immunoprecipitation with the VPS26A antibody (2.5 µg, **VPA00872**); the IgG-IP lane is the immunoprecipitation with Rabbit polyclonal IgG(2.5 µg, PRABP01). Western blot analysis was performed using VPS26A antibody (1/500, **VPA00872**) followed by detection with HRP conjugated TidyBlot Western Blot Detection Reagent (1/400, STAR209) and visualized on the ChemiDoc MP. Arrow points to VPS26A.

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Rabbit anti VPS26A

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: VPS26A

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00872 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Rabbit anti Human VPS26A antibody recognizes VPS26A, also known as vacuolar protein sorting-associated protein 26A and vesicle protein sorting 26A.

VPS26A belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

Rabbit anti Human VPS26A antibody detects a band of 38 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti VPS26A antibody recognizes a band of approximately 38 kDa in HEK293 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
2% Sucrose
Immunogen: Synthetic peptide corresponding to the C terminal segment of human VPS26A
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: O75436
Entrez Gene: VPS26A
GO Terms: GO:0005515 protein binding; GO:0005829 cytosol; GO:0007034 vacuolar transport; GO:0008565 protein transporter activity; GO:0010008 endosome membrane; GO:0030904 retromer complex; GO:0042147 retrograde transport, endosome to Golgi