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Vitamin D Receptor antibody

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Anti Human Vitamin D Receptor Antibody gallery image 1
Western blotting analysis of Human Brain lysate (35μg protein in RIPA buffer) using Goat anti Human vitamin D receptor antibody (AHP1093) at 0.3μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Goat anti Human Vitamin D Receptor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1093
Datasheet
E WB
SDS
0.1 mg

Goat anti Human Vitamin D Receptor antibody recognises human vitamin D nuclear hormone receptor (VDR) a ~48 kDa member of the trans-acting transcriptional regulatory factors. On binding vitamin D, VDR regulates the expression of hormone sensitive genes. The main downstream target of VDR is involved in bone mineral metabolism although VDR is also involved in immune response and cancer metabolic pathways. VDR also acts as a receptor for secondary bile acid lithocholic acid. Additionally, signalling and oncogenic activity of beta-catenin signalling has been shown to be repressed by VDR (Shah et al. 2006).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human Vitamin D receptor were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence CGNQDYKYRVSD corresponding to the internal region of Vitamin D receptor (NP_000367.1).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P11473
Entrez Gene
VDR
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0005654 nucleoplasm
GO:0008270 zinc ion binding
GO:0008285 negative regulation of cell proliferation
GO:0008434 vitamin D3 receptor activity
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010980 positive regulation of vitamin D 24-hydroxylase activity
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0046697 decidualization
GO:0046965 retinoid X receptor binding
GO:0060558 regulation of calcidiol 1-monooxygenase activity
Regulatory
For research purposes only

Applications of Vitamin D Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16,000
Western Blotting 0.3 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1093 detects a band of approximately 40-45kDa in human brain cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for Vitamin D Receptor antibody

  1. Cheng, H.T. et al. (2006) Functional role of VDR in the activation of p27Kip1 by the VDR/Sp1 complex.
    J Cell Biochem. 98 (6): 1450-6.

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