VDAC1 antibody
Rabbit anti VDAC1
VDAC1 antibody recognizes voltage-dependent anion-selective channel protein 1, also known as outer mitochondrial membrane protein porin 1, plasmalemmal porin, porin 31HL, or porin 31HM. This antibody has been extensively validated for western blotting using whole cell lysates.
VDAC1 Antibody with Control Lysate
VDAC1 antibody recognizes voltage-dependent anion-selective channel protein 1, also known as outer mitochondrial membrane protein porin 1, plasmalemmal porin, porin 31HL, or porin 31HM. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00169 | WB | 100 µl | ||||
| VPA00169KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human VDAC1 antibody recognizes the voltage-dependent anion-selective channel protein 1, also known as outer mitochondrial membrane protein porin 1, plasmalemmal porin, porin 31HL, porin 31HM and voltage-dependent anion-selective channel protein 1.
VDAC1 is a voltage-dependent anion channel protein and a major component of the outer mitochondrial membrane. This protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. It also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of VDAC1 are found on chromosomes 1, 2 3, 6, 9, 12, X and Y (provided by RefSeq, Sep 2010).
Rabbit anti Human VDAC1 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
VDAC1 is a voltage-dependent anion channel protein and a major component of the outer mitochondrial membrane. This protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. It also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of VDAC1 are found on chromosomes 1, 2 3, 6, 9, 12, X and Y (provided by RefSeq, Sep 2010).
Rabbit anti Human VDAC1 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti VDAC1 detects a band of approximately 32 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids 185-197 of human VDAC1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P21796 Related reagents
- Entrez Gene
- VDAC1 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0005741 mitochondrial outer membrane
- GO:0008308 voltage-gated anion channel activity
- GO:0015288 porin activity
- GO:0046930 pore complex
- GO:0042645 mitochondrial nucleoid
- GO:0044419 interspecies interaction between organisms
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of VDAC1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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