Rabbit anti VAV3 antibody
recognizes guanine nucleotide exchange factor, also known as VAV-3. It belongs to the VAV family of proteins; the main function of VAV proteins is to work as guanosine nucleotide exchange factors (GEFs) for members of the Rho GTPase family (Bustelo 2014
). VAV3 was initially detected in expressed sequence tag (EST) libraries (Movilla and Bustelo 1999
). It mediates receptor protein tyrosine kinase signaling (Zeng et al. 2000
). Protein overexpression of VAV3 has been reported in several types of human cancer, including breast cancer (Lee et al. 2008
) and prostate cancer (Dong et al. 2006
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to VAV3 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant fusion protein containing a sequence corresponding to amino acids 560-680 of human VAV3
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
SH3/SH2 adaptor activity
GTPase activator activity
metal ion binding
response to DNA damage stimulus
small GTPase mediated signal transduction
induction of apoptosis by extracellular signals
response to drug
positive regulation of B cell proliferation
regulation of Rho protein signal transduction
nerve growth factor receptor signaling pathway
B cell receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of VAV3 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti VAV3 antibody detects a band of approximately 105 kDa in Jurkat cell lysate and mouse tissue lysates under reducing conditions
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Secondary Antibodies Available