VAV3 antibody
Rabbit anti VAV3 antibody recognizes guanine nucleotide exchange factor, also known as VAV-3. It belongs to the VAV family of proteins; the main function of VAV proteins is to work as guanosine nucleotide exchange factors (GEFs) for members of the Rho GTPase family (Bustelo 2014). VAV3 was initially detected in expressed sequence tag (EST) libraries (Movilla and Bustelo 1999). It mediates receptor protein tyrosine kinase signaling (Zeng et al. 2000). Protein overexpression of VAV3 has been reported in several types of human cancer, including breast cancer (Lee et al. 2008) and prostate cancer (Dong et al. 2006).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to VAV3 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 560-680 of human VAV3
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9UKW4
- Entrez Gene
- VAV3
- GO Terms
- GO:0001525 angiogenesis
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0005070 SH3/SH2 adaptor activity
- GO:0005096 GTPase activator activity
- GO:0046872 metal ion binding
- GO:0006974 response to DNA damage stimulus
- GO:0007264 small GTPase mediated signal transduction
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0042493 response to drug
- GO:0030168 platelet activation
- GO:0030890 positive regulation of B cell proliferation
- GO:0035023 regulation of Rho protein signal transduction
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- Regulatory
- For research purposes only
Applications of VAV3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/200 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti VAV3 antibody detects a band of approximately 105 kDa in Jurkat cell lysate and mouse tissue lysates under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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