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VAV1 (pTyr174) antibody

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Anti VAV1 (pTyr174) Antibody gallery image 1
Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using Rabbit anti VAV1 (pTyr174) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with H2O2 (2mM) for 15 minutes

Rabbit anti VAV1 (pTyr174)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2691
Datasheet
WB
SDS
50 µl loader

Rabbit anti VAV1 (pTyr174) antibody recognizes VAV1, also known as vav guanine nucleotide exchange factor 1 when phosphorylated at tyrosine 174. VAV1 is a member of the Vav family of guanine nucleotide exchange factors (GEFs). GEFs are involved in the activation of small GTPases which play a role in many signaling pathways. Potential substrates for VAV1 activity include Rac1, Cdc42 RhoA and RhoG. VAV1 is phosphorylated on tyrosine 174 leads to conformational changes and activation of VAV1 (Katzav 2015).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to VAV1 (pTyr174) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 174 of human VAV1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P15498
Entrez Gene
VAV1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005089 Rho guanyl-nucleotide exchange factor activity
GO:0046872 metal ion binding
GO:0007264 small GTPase mediated signal transduction
GO:0008624 induction of apoptosis by extracellular signals
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0035023 regulation of Rho protein signal transduction
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of VAV1 (pTyr174) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Katzav, S. (2015) Vav1: A Dr. Jekyll and Mr. Hyde protein--good for the hematopoietic system, bad for cancer.
    Oncotarget. 6 (30): 28731-42.

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