VASP (pSer157) antibody

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Anti VASP (pSer157) Antibody thumbnail image 2

Anti VASP (pSer157) Antibody gallery image 1 — Western blot analysis of extracts from A431 cells untreated or treated with Forskolin using Rabbit anti VASP (pSer157) antibody at a 1/2000 dilution. Cells were cultured in serum free media overnight before treatment with Forskolin (10μM) for 30 minutes. 3% BSA was used for blocking.

Anti VASP (pSer157) Antibody gallery image 2 — Western blot analysis of VASP (pSer157) IP samples. IP was performed on 200 μg A431 cells lysate treated by forskolin using 2.5 μg Rabbit anti VASP (pSer157) antibody. Western blot was performed on the immunoprecipitates using Rabbit anti VASP (pSer157) antibody at a dilution of 1/1000.

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Rabbit anti VASP (pSer157)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: VASP (pSer157)

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AHP2701 Datasheet Datasheet SDS SDS	IP WB	50 µl		Log in	Get Quote
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Rabbit anti VASP (pSer157) antibody recognizes vasodilator-stimulated phosphoprotein (VASP), when phosphorylated at serine 157. VASP belongs to the Ena/VASP family and is associated with actin filaments and focal adhesions. It is involved in the remodeling of the actin cytoskeleton and platelet aggregation (Machesky, L.M. 2000, Krause, M. et al. 2003 & Aszodi, A. et al. 1999). In platelets VASP is phosphorylated on serine 157 by PKC and Rho kinases in response to thrombin (Wentworth, J.K. et al. 2006). Phosphorylation of VASP regulates its interaction with actin (Harbeck, B. et al. 2000). In smooth muscle cells, phosphorylation of VASP on serine 157 promotes proliferation (Chen, L. et al. 2004).

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to VASP (pSer157) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Phospho specific-peptide corresponding to residues surrounding serine 157 of human VASP
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation		1/50	1/100
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt: P50552
Entrez Gene: VASP
GO Terms: GO:0003779 actin binding; GO:0005829 cytosol; GO:0005522 profilin binding; GO:0005925 focal adhesion; GO:0007411 axon guidance; GO:0015629 actin cytoskeleton; GO:0017124 SH3 domain binding; GO:0031258 lamellipodium membrane; GO:0031527 filopodium membrane; View More GO Terms; GO:0034329 cell junction assembly; GO:0050852 T cell receptor signaling pathway