VASP (pSer157) antibody

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Rabbit anti VASP (pSer157)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2701
Datasheet
IP WB
MSDS
50 µl loader

Rabbit anti VASP (pSer157) antibody recognizes vasodilator-stimulated phosphoprotein (VASP), when phosphorylated at serine 157. VASP belongs to the Ena/VASP family and is associated with actin filaments and focal adhesions. It is involved in the remodeling of the actin cytoskeleton and platelet aggregation (Machesky, L.M. 2000, Krause, M. et al. 2003 & Aszodi, A. et al. 1999). In platelets VASP is phosphorylated on serine 157 by PKC and Rho kinases in response to thrombin (Wentworth, J.K. et al. 2006). Phosphorylation of VASP regulates its interaction with actin (Harbeck, B. et al. 2000). In smooth muscle cells, phosphorylation of VASP on serine 157 promotes proliferation (Chen, L. et al. 2004).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to VASP (pSer157) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 157 of human VASP

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P50552 Related reagents
Entrez Gene
VASP Related reagents
GO Terms
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005522 profilin binding
GO:0005925 focal adhesion
GO:0007411 axon guidance
GO:0015629 actin cytoskeleton
GO:0017124 SH3 domain binding
GO:0031258 lamellipodium membrane
GO:0031527 filopodium membrane
GO:0034329 cell junction assembly
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of VASP (pSer157) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti VASP VMA00326 WB 100 µl loader
Rabbit anti VASP VPA00347 WB 100 µl loader