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VASP (pSer157) antibody

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Anti VASP (pSer157) Antibody thumbnail image 1 Anti VASP (pSer157) Antibody thumbnail image 2
Anti VASP (pSer157) Antibody gallery image 1
Western blot analysis of extracts from A431 cells untreated or treated with Forskolin using Rabbit anti VASP (pSer157) antibody at a 1/2000 dilution. Cells were cultured in serum free media overnight before treatment with Forskolin (10μM) for 30 minutes. 3% BSA was used for blocking.
Anti VASP (pSer157) Antibody gallery image 2
Western blot analysis of VASP (pSer157) IP samples. IP was performed on 200 μg A431 cells lysate treated by forskolin using 2.5 μg Rabbit anti VASP (pSer157) antibody. Western blot was performed on the immunoprecipitates using Rabbit anti VASP (pSer157) antibody at a dilution of 1/1000.

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WB IP

Rabbit anti VASP (pSer157)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
VASP (pSer157)

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AHP2701
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SDS Safety Datasheet SDS
IP WB 50 µl loader
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Rabbit anti VASP (pSer157) antibody recognizes vasodilator-stimulated phosphoprotein (VASP), when phosphorylated at serine 157. VASP belongs to the Ena/VASP family and is associated with actin filaments and focal adhesions. It is involved in the remodeling of the actin cytoskeleton and platelet aggregation (Machesky, L.M. 2000, Krause, M. et al. 2003 & Aszodi, A. et al. 1999). In platelets VASP is phosphorylated on serine 157 by PKC and Rho kinases in response to thrombin (Wentworth, J.K. et al. 2006). Phosphorylation of VASP regulates its interaction with actin (Harbeck, B. et al. 2000). In smooth muscle cells, phosphorylation of VASP on serine 157 promotes proliferation (Chen, L. et al. 2004).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to VASP (pSer157) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 157 of human VASP
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P50552
Entrez Gene
VASP
GO Terms
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005522 profilin binding
GO:0005925 focal adhesion
GO:0007411 axon guidance
GO:0015629 actin cytoskeleton
GO:0017124 SH3 domain binding
GO:0031258 lamellipodium membrane
GO:0031527 filopodium membrane
GO:0034329 cell junction assembly
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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