VASP (pSer157) antibody
Rabbit anti VASP (pSer157) antibody recognizes vasodilator-stimulated phosphoprotein (VASP), when phosphorylated at serine 157. VASP belongs to the Ena/VASP family and is associated with actin filaments and focal adhesions. It is involved in the remodeling of the actin cytoskeleton and platelet aggregation (Machesky, L.M. 2000, Krause, M. et al. 2003 & Aszodi, A. et al. 1999). In platelets VASP is phosphorylated on serine 157 by PKC and Rho kinases in response to thrombin (Wentworth, J.K. et al. 2006). Phosphorylation of VASP regulates its interaction with actin (Harbeck, B. et al. 2000). In smooth muscle cells, phosphorylation of VASP on serine 157 promotes proliferation (Chen, L. et al. 2004).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to VASP (pSer157) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 157 of human VASP
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P50552
- Entrez Gene
- VASP
- GO Terms
- GO:0003779 actin binding
- GO:0005829 cytosol
- GO:0005522 profilin binding
- GO:0005925 focal adhesion
- GO:0007411 axon guidance
- GO:0015629 actin cytoskeleton
- GO:0017124 SH3 domain binding
- GO:0031258 lamellipodium membrane
- GO:0031527 filopodium membrane
- GO:0034329 cell junction assembly
- GO:0050852 T cell receptor signaling pathway
- Regulatory
- For research purposes only
Applications of VASP (pSer157) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti VASP | VMA00326 | WB | 100 µl |
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| Rabbit anti VASP | VPA00347 | WB | 100 µl |
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