USP7 antibody

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Rabbit anti USP7

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1937
Datasheet
E IP P WB
MSDS
50 µg loader

Rabbit anti Human USP7 antibody recognizes human Ubiquitin carboxyl-terminal hydrolase 7, also known asUSP7 or HAUSP. USP7 is a ~128 kDa ubiquitin specific protease belonging to the peptidase C19 family.

USP7 is deubiquitylating enzyme that cleaves ubiquitin from its substrates resulting in stabilization of the protein and is a direct antagonist of MDM2 and has been demonstrated to protect p53 from degradation by MDM2 and thus implying that USP7 may act in a tumour suppressor role.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Zebrafish
Chimpanzee
Dog
Chicken
Rat
Mouse
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human USP7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to amino acids 30-41 of human USP7.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q93009 Related reagents
Entrez Gene
USP7 Related reagents
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0002039 p53 binding
GO:0004221 ubiquitin thiolesterase activity
GO:0004843 ubiquitin-specific protease activity
GO:0005737 cytoplasm
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007275 multicellular organismal development
GO:0008022 protein C-terminus binding
GO:0008134 transcription factor binding
GO:0016579 protein deubiquitination
GO:0016605 PML body
GO:0031625 ubiquitin protein ligase binding
GO:0044419 interspecies interaction between organisms
GO:0051090 regulation of transcription factor activity
Regulatory
For research purposes only

Applications of USP7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1:15000 1:60000
Immunohistology - Paraffin 5ug/ml
Immunoprecipitation
Western Blotting 1:500 1:2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Human skeletal muscle

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Histar Detection System STAR3000A C P 50 Tests