USP2 antibody

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Rabbit anti Human USP2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1154 P* 50 µg
Rabbit anti Human USP2 antibody detects an epitope within the internal region of human ubiquitin specific protease 2 (USP2), also known as UBP41. USP2 is a deubiquitinating protease which rescues proteins from the ubiquitin mediated 26S proteasomal pathway. The USP2 gene encodes two splice variants- USP2a and USP2b, with molecular weights of ~69 kDa and ~48 kDa respectively.

USP2a deubiquitinates the antiapoptotic proteins fatty acid synthase (FAS) and Mdm2. FAS is overexpressed in the majority of epithelial tumors, including prostate cancer, whilst Mdm2 indicates that USP2 may regulate p53-dependent pathways.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to USP2 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an internal domain of human USP2-conjugated to KLH.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O75604 Related reagents
Entrez Gene
USP2 Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007049 cell cycle
GO:0007517 muscle organ development
GO:0016579 protein deubiquitination
GO:0050821 protein stabilization
GO:0030332 cyclin binding
GO:0031625 ubiquitin protein ligase binding
GO:0045931 positive regulation of mitotic cell cycle
GO:0048471 perinuclear region of cytoplasm
Regulatory
For research purposes only

Applications of USP2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1 5ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Skeletal muscle.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

Further Reading

  1. Stevenson, L.F. et al. (2007) The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2.
    EMBO J. 26 (4): 976-86.
  2. Priolo, C. et al. (2006) The isopeptidase USP2a protects human prostate cancer from apoptosis.
    Cancer Res. 66 (17): 8625-32.
  3. Graner, E. et al. (2004) The isopeptidase USP2a regulates the stability of fatty acid synthase in prostate cancer.
    Cancer Cell. 5 (3): 253-61.
  4. Gewies, A. & Grimm, S. (2003) UBP41 is a proapoptotic ubiquitin-specific protease.
    Cancer Res. 63 (3): 682-8.