Rabbit anti Human USP2 antibody detects an epitope within the internal region of human ubiquitin specific protease 2 (USP2), also known as UBP41. USP2 is a deubiquitinating protease which rescues proteins from the ubiquitin mediated 26S proteasomal pathway. The USP2 gene encodes two splice variants- USP2a and USP2b, with molecular weights of ~69 kDa and ~48 kDa respectively.
USP2a deubiquitinates the antiapoptotic proteins fatty acid synthase (FAS) and Mdm2. FAS is overexpressed in the majority of epithelial tumors, including prostate cancer, whilst Mdm2 indicates that USP2 may regulate p53-dependent pathways.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to USP2 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Synthetic peptide corresponding to an internal domain of human USP2-conjugated to KLH.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of transcription from RNA polymerase II promoter
cysteine-type endopeptidase activity
ubiquitin thiolesterase activity
metal ion binding
ubiquitin-dependent protein catabolic process
muscle organ development
ubiquitin protein ligase binding
positive regulation of mitotic cell cycle
perinuclear region of cytoplasm
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of USP2 antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Skeletal muscle.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Stevenson, L.F. et al. (2007) The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2.
EMBO J. 26 (4): 976-86.
Priolo, C. et al. (2006) The isopeptidase USP2a protects human prostate cancer from apoptosis.
Cancer Res. 66 (17): 8625-32.
Graner, E. et al. (2004) The isopeptidase USP2a regulates the stability of fatty acid synthase in prostate cancer.
Cancer Cell. 5 (3): 253-61.
Gewies, A. & Grimm, S. (2003) UBP41 is a proapoptotic ubiquitin-specific protease.
Cancer Res. 63 (3): 682-8.