USP14 antibody

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Rabbit anti USP14

Product Type
PrecisionAb Polyclonal
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
100 µl loader

Rabbit anti Human USP14 antibody recognizes the ubiquitin carboxyl-terminal hydrolase 14, also known as deubiquitinating enzyme 14, tRNA-guanine transglycosylase, 60-kD subunit, ubiquitin carboxyl-terminal hydrolase 14, ubiquitin specific protease 14 (tRNA-guanine transglycosylase), ubiquitin thioesterase 14, ubiquitin thiolesterase 14, ubiquitin-specific processing protease 14 or ubiquitin-specific-processing protease 14.

The USP14 gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human USP14 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Western Blotting
Rabbit anti USP14 detects a band of approximately 60 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Synthetic peptide encompassing part of the middle region of human USP14

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0000502 proteasome complex
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0004843 ubiquitin-specific protease activity
GO:0004866 endopeptidase inhibitor activity
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0008193 tRNA guanylyltransferase activity
GO:0061136 regulation of proteasomal protein catabolic process
GO:0009986 cell surface
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0050920 regulation of chemotaxis
GO:0070628 proteasome binding
PrecisionAb is a trademark of Bio-Rad Laboratories
For research purposes only

Applications of USP14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml