Find out more about A.I.den - our SearchBot

Urokinase antibody

100% Secure
Anti Human Urokinase (C-Terminal) Antibody gallery image 1
Western blotting analysis of HEK293 lysate (35μg protein in RIPA buffer) using Goat anti Human urokinase antibody (AHP1065) at 0.3μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Goat anti Human Urokinase (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1065
Datasheet
E WB
SDS
0.1 mg

Goat anti Human Urokinase antibody detects an epitope within the C-terminal of urokinase, a potent ~50 kDa serine protease also known as urokinase plasminogen activator (uPA). uPA cleaves and activates plasminogen to form plasmin and is involved in extracellular cellular matrix degradation and cell signalling. Recent studies have implicated uPA and its receptor in the pathogenesis of inflammation and immunity.

A specific polymorphism in the gene encoding uPA may be associated with late-onset Alzheimer's disease (Ertekin-Taner et al. 2005). The levels of uPA, uPAR and its soluble fragments have also been found to be increased in tissues, plasma and other body fluids of cancer patients and to be markers of cancer development and metastasis (Kim et al. 2016).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Urokinase were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence C-HFLPWIRSHTKEEN from the C-terminal of the Urokinase sequence.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P00749
Entrez Gene
PLAU
GO Terms
GO:0005886 plasma membrane
GO:0004252 serine-type endopeptidase activity
GO:0007596 blood coagulation
GO:0007165 signal transduction
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0006935 chemotaxis
GO:0009986 cell surface
GO:0010469 regulation of receptor activity
GO:0014910 regulation of smooth muscle cell migration
GO:0033628 regulation of cell adhesion mediated by integrin
GO:0042730 fibrinolysis
GO:2000097 regulation of smooth muscle cell-matrix adhesion
Regulatory
For research purposes only

Applications of Urokinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000
Western Blotting 0.3 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1065 detects a band of approximately 48kDa in KEH 293 cell lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for Urokinase antibody

  1. Mondino, A. & Blasi, F. (2004) uPA and uPAR in fibrinolysis, immunity and pathology.
    Trends Immunol. 25 (8): 450-5.
  2. Danø, K. et al. (2005) Plasminogen activation and cancer.
    Thromb Haemost. 93 (4): 676-81.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra
Request a different product with the Urokinase specificity