Urokinase antibody
Goat anti Human Urokinase antibody detects an epitope within the C-terminal of urokinase, a potent ~50 kDa serine protease also known as urokinase plasminogen activator (uPA). uPA cleaves and activates plasminogen to form plasmin and is involved in extracellular cellular matrix degradation and cell signalling. Recent studies have implicated uPA and its receptor in the pathogenesis of inflammation and immunity.
A specific polymorphism in the gene encoding uPA may be associated with late-onset Alzheimer's disease (Ertekin-Taner et al. 2005). The levels of uPA, uPAR and its soluble fragments have also been found to be increased in tissues, plasma and other body fluids of cancer patients and to be markers of cancer development and metastasis (Kim et al. 2016).
A specific polymorphism in the gene encoding uPA may be associated with late-onset Alzheimer's disease (Ertekin-Taner et al. 2005). The levels of uPA, uPAR and its soluble fragments have also been found to be increased in tissues, plasma and other body fluids of cancer patients and to be markers of cancer development and metastasis (Kim et al. 2016).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Urokinase were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin - Immunogen
- Peptide with sequence C-HFLPWIRSHTKEEN from the C-terminal of the Urokinase sequence.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00749
- Entrez Gene
- PLAU
- GO Terms
- GO:0005886 plasma membrane
- GO:0004252 serine-type endopeptidase activity
- GO:0007596 blood coagulation
- GO:0007165 signal transduction
- GO:0005615 extracellular space
- GO:0006508 proteolysis
- GO:0006935 chemotaxis
- GO:0009986 cell surface
- GO:0010469 regulation of receptor activity
- GO:0014910 regulation of smooth muscle cell migration
- GO:0033628 regulation of cell adhesion mediated by integrin
- GO:0042730 fibrinolysis
- GO:2000097 regulation of smooth muscle cell-matrix adhesion
- Regulatory
- For research purposes only
Applications of Urokinase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/8000 | ||
| Western Blotting | 0.3 | 1.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1065 detects a band of approximately 48kDa in KEH 293 cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for Urokinase antibody
-
Mondino, A. & Blasi, F. (2004) uPA and uPAR in fibrinolysis, immunity and pathology.
Trends Immunol. 25 (8): 450-5. -
Danø, K. et al. (2005) Plasminogen activation and cancer.
Thromb Haemost. 93 (4): 676-81.
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