Uracil-DNA Glycosylase 1 antibody

Rabbit anti Uracil-DNA Glycosylase 1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Uracil-DNA Glycosylase 1
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1201
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
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loader
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Rabbit anti Uracil-DNA glycolase 1 antibody recognizes uracil-DNA glycosylase 1 (UNG1), a member of the uracil-DNA glycosylase and cyclin family. Two isoforms of UNG exist, isoform 1 is expressed in the mitochondria of in skeletal muscle, heart and testicles while isoform 2 (UNG2) is more abundant in proliferating tissues and expressed in the nucleus.

Uracil residues can be misincorporated into DNA by DNA polymerase or by deamination of cytosine. UNG1 is a major enzyme in base excision repair, excising the misincorporated uracil residues in a process crucial to increasing immunoglobulin diversity. In association with activation-induced cytosine deaminase, UNG is also essential to the generation of strand breaks that initiate class switch recombination.

UNG is also involved in the innate immune response against retroviral infections, HIV-1 accessory protein Vpr induces the rapid degradation of UNG2.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human uracil-DNA glycosylase 1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
14 amino acid peptide sequence near the amino terminus of human UNG1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1201 detects a band of approximately 34.6kDa in C2C/2 cell lysates.

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Further Reading

  1. Hagen, L. et al. (2007) Genomic uracil and human disease.
    Exp Cell Res. 312:2666-2672.
  2. Krokan, H.E. et al. (2001) Properties and functions of human uracil-DNA glycosylase from the UNG gene.
    Prog Nucleic Acid Res Mol Biol. 68: 365-86.
  3. Akbari, M. et al. (2007) Different organization of base excision repair of uracil in DNA in nuclei and mitochondria and selective upregulation of mitochondrial uracil-DNA glycosylase after oxidative stress.
    Neuroscience. 145 (4): 1201-12.

Synonyms
UNG1
RRID
AB_844685
UniProt
P13051
Entrez Gene
UNG
GO Terms
GO:0005515 protein binding
GO:0005739 mitochondrion
GO:0004844 uracil DNA N-glycosylase activity
GO:0005634 nucleus
GO:0006284 base-excision repair
GO:0044419 interspecies interaction between organisms
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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