Uracil-DNA Glycosylase 1 antibody


Uracil residues can be misincorporated into DNA by DNA polymerase or by deamination of cytosine. UNG1 is a major enzyme in base excision repair, excising the misincorporated uracil residues in a process crucial to increasing immunoglobulin diversity. In association with activation-induced cytosine deaminase, UNG is also essential to the generation of strand breaks that initiate class switch recombination.
UNG is also involved in the innate immune response against retroviral infections, HIV-1 accessory protein Vpr induces the rapid degradation of UNG2.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human uracil-DNA glycosylase 1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- 14 amino acid peptide sequence near the amino terminus of human UNG1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P13051
- Entrez Gene
- UNG
- GO Terms
- GO:0005515 protein binding
- GO:0005739 mitochondrion
- GO:0004844 uracil DNA N-glycosylase activity
- GO:0005634 nucleus
- GO:0006284 base-excision repair
- GO:0044419 interspecies interaction between organisms
- Regulatory
- For research purposes only
Applications of Uracil-DNA Glycosylase 1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 0.5 | 1.0ug/ml |
- Western Blotting
- AHP1201 detects a band of approximately 34.6kDa in C2C/2 cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Hagen, L. et al. (2007) Genomic uracil and human disease.
Exp Cell Res. 312:2666-2672. -
Krokan, H.E. et al. (2001) Properties and functions of human uracil-DNA glycosylase from the UNG gene.
Prog Nucleic Acid Res Mol Biol. 68: 365-86. -
Akbari, M. et al. (2007) Different organization of base excision repair of uracil in DNA in nuclei and mitochondria and selective upregulation of mitochondrial uracil-DNA glycosylase after oxidative stress.
Neuroscience. 145 (4): 1201-12.
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