UQCRC2 antibody
Rabbit anti UQCRC2
UQCRC2 antibody recognizes UQCRC2, also known as complex III subunit 2 core protein II or ubiquinol-cytochrome-c reductase complex core protein 2.
UQCRC2 Antibody with Control Lysate
UQCRC2 antibody recognizes UQCRC2, also known as complex III subunit 2 core protein II or ubiquinol-cytochrome-c reductase complex core protein 2.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00871 | WB | 100 µl | ||||
| VPA00871KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human UQCRC2 antibody recognizes UQCRC2, also known as complex III subunit 2 core protein II, cytochrome b-c1 complex subunit 2-mitochondrial and ubiquinol-cytochrome-c reductase complex core protein 2.
Rabbit anti Human UQCRC2 antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Rabbit anti Human UQCRC2 antibody detects a band of 48 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti UQCRC2 antibody recognizes a band of approximately 48 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the middle segment of human UQCRC2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P22695 Related reagents
- Entrez Gene
- UQCRC2 Related reagents
- GO Terms
- GO:0004222 metalloendopeptidase activity
- GO:0022904 respiratory electron transport chain
- GO:0006119 oxidative phosphorylation
- GO:0006508 proteolysis
- GO:0006810 transport
- GO:0008270 zinc ion binding
- GO:0009060 aerobic respiration
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of UQCRC2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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