Uncoupling Protein 4 antibody

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Rabbit anti Human Uncoupling Protein 4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0100-0335 E WB 1 ml
Rabbit anti Human Uncoupling Protein 4 antibody recognizes human mitochondrial uncoupling protein 4, also known as uncoupling Protein 4, UCP 4, Solute carrier family 25 member 27 or SLC25A27. Uncoupling protein 4 is a 323 amino acid, ~36kDa multiple pass, inner mitochondrial transmembrane protein bearing three Solcar repeats (UniProt: O95847)

Uncoupling protein expression appears to be primarily associated with mitochondria of the central nervous syatem, with no protein being found in a number of peripheral organs when tested in immunohistochemistry, or western blotting in mice and rats. In mice uncoupling protein 4 appears in early to mid embryonic development coinciding with the initiation of neuronal differentiation and expression in mitochondria decreases with age, hinting at a possible role in neuronal differentiation (Smorodchenko et al. 2009). UCP4 facilitates the dissipation of heat across the inner mitochondrial membrane, by leaking protons across the membrane and uncoupling oxidative phosphorylation from ATP synthesis (Mao et al. 1999), the action of uncoupling protein 4 increasing proton concentration in the mitochondrial matrix may thus serve to increase ATP production by the class II complex (Ho et al. 2012). UCP4 is understood to be involved in thermoregulatory heat production and metabolism in brain.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the amino acids 271 - 283 of the native molecule (KSSTDCLIQA VQG) conjugated to Keyhole Limpet Haemocyanin.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O95847 Related reagents
Entrez Gene
SLC25A27 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005488 binding
GO:0005743 mitochondrial inner membrane
GO:0006091 generation of precursor metabolites and energy
GO:0055085 transmembrane transport
Regulatory
For research purposes only

Applications of Uncoupling Protein 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
In reduced blots on bovine brown adipose tissue homogenates, this antibody recognises specific bands at ~34 & 44 kDa and other bands at ~50 & 60 kDa that can be blocked with KLH.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*