UBIQUILIN-1 antibody

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Rabbit anti UBIQUILIN-1

Rabbit anti-human ubiquilin-1 antibody recognizes ubiquilin-1, also known as hPLIC-1 or protein linking IAP with cytoskeleton 1. This antibody has been extensively validated for western blotting using whole cell lysates.

UBIQUILIN-1 Antibody with Control Lysate

Rabbit anti-human ubiquilin-1 antibody recognizes ubiquilin-1, also known as hPLIC-1 or protein linking IAP with cytoskeleton 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00453 WB 100 µl
VPA00453KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human ubiquilin-1 antibody recognizes ubiquilin-1, also known as hPLIC-1 or protein linking IAP with cytoskeleton 1.

The UBQLN1 gene encodes an ubiquitin-like protein (ubiquilin) that shares a high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to modulate accumulation of presenilin proteins, and it is found in lesions associated with Alzheimer's and Parkinson's disease (Mah et al. 2000). Two transcript variants encoding different isoforms have been found for UBQLN1 (provided by RefSeq, Jul 2008).

Rabbit anti Human ubiquilin-1 antibody detects a band of 66 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the middle region of human ubiquilin-1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9UMX0 Related reagents
Entrez Gene
UBQLN1 Related reagents
GO Terms
GO:0000502 proteasome complex
GO:0001666 response to hypoxia
GO:0006915 apoptosis
GO:0005783 endoplasmic reticulum
GO:0005634 nucleus
GO:0019900 kinase binding
GO:0031396 regulation of protein ubiquitination
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UBIQUILIN-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti Ubiquilin-1 detects a band of approximately 66 kDa in HEK293 cell lysates
Western Blotting
Anti Ubiquilin-1 detects a band of approximately 66 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB