UBE4B antibody

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Rabbit anti UBE4B

Rabbit anti human UBE4B antibody recognizes ubiquitin conjugation factor E4B, also known as UBOX3, UFD2A-III/UBE4B-III splice isoform, homologous to yeast UFD2, or ubiquitination factor E4B (homologous to yeast UFD2). This antibody has been validated for western blotting using whole cell lysates.

UBE4B Antibody with Control Lysate

Rabbit anti human UBE4B antibody recognizes ubiquitin conjugation factor E4B, also known as UBOX3, UFD2A-III/UBE4B-III splice isoform, homologous to yeast UFD2, homozygously deleted in neuroblastoma 1, ubiquitin conjugation factor E4B, or ubiquitin fusion degradation protein 2.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00346 WB 100 µl
VPA00346KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human UBE4B antibody recognizes UBE4B, also known as UBOX3, UFD2A-III/UBE4B-III splice isoform, homologous to yeast UFD2, homozygously deleted in neuroblastoma-1, ubiquitin conjugation factor E4B, ubiquitin-fusion degradation protein 2 and ubiquitination factor E4B (homologous to yeast UFD2).

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. The UBE4B gene encodes an additional conjugation factor, E4, which is involved in multi-ubiquitin chain assembly. UBE4B is also the strongest neuroblastoma tumor suppressor gene. Alternatively spliced transcript variants encoding distinct isoforms have been found for UBE4B (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE4B antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti UBE4B detects a band of approximately 140 kDa in K562 cell lysate
Product Form
Ig fraction - liquid
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 112-142 of human UBE4B
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O95155 Related reagents
Entrez Gene
UBE4B Related reagents
GO Terms
GO:0000151 ubiquitin ligase complex
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0006915 apoptosis
GO:0005737 cytoplasm
GO:0009411 response to UV
GO:0019899 enzyme binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UBE4B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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