UBE4B antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti UBE4B Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with UBE4B antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to UBE4B (molecular weight 140 kDa)

Filter by Application:

Rabbit anti UBE4B

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: UBE4B

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00346 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Rabbit anti Human UBE4B antibody recognizes UBE4B, also known as UBOX3, UFD2A-III/UBE4B-III splice isoform, homologous to yeast UFD2, homozygously deleted in neuroblastoma-1, ubiquitin conjugation factor E4B, ubiquitin-fusion degradation protein 2 and ubiquitination factor E4B (homologous to yeast UFD2).

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. The UBE4B gene encodes an additional conjugation factor, E4, which is involved in multi-ubiquitin chain assembly. UBE4B is also the strongest neuroblastoma tumor suppressor gene. Alternatively spliced transcript variants encoding distinct isoforms have been found for UBE4B (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE4B antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti UBE4B detects a band of approximately 140 kDa in K562 cell lysate.
Product Form: Ig fraction - liquid
Preparation: Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH-conjugated synthetic peptide corresponding to aa 112-142 of human UBE4B
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: O95155
Entrez Gene: UBE4B
GO Terms: GO:0000151 ubiquitin ligase complex; GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process; GO:0006915 apoptosis; GO:0005737 cytoplasm; GO:0009411 response to UV; GO:0019899 enzyme binding