UBE2I antibody
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes or E1s, ubiquitin-conjugating enzymes or E2s, and ubiquitin-protein ligases or E3s. UBE2I encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for UBE2I (provided by RefSeq, Jul 2008).
Rabbit anti Human UBE2I antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti UBE2I antibody recognizes a band of approximately 17 kDa in HT-29 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the N terminal region of human UBE2I
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P63279
- Entrez Gene
- UBE2I
- GO Terms
- GO:0007059 chromosome segregation
- GO:0000795 synaptonemal complex
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0005737 cytoplasm
- GO:0006511 ubiquitin-dependent protein catabolic process
- GO:0007067 mitosis
- GO:0008134 transcription factor binding
- GO:0016566 specific transcriptional repressor activity
- GO:0016605 PML body
- GO:0016925 protein sumoylation
- GO:0019899 enzyme binding
- GO:0043687 post-translational protein modification
- GO:0044419 interspecies interaction between organisms
- GO:0045892 negative regulation of transcription, DNA-dependent
- GO:0051246 regulation of protein metabolic process
- GO:0051301 cell division
- GO:0072500 negative regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of UBE2I antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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