UBE2I antibody

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Rabbit anti UBE2I

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00791
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human UBE2I antibody recognizes the ubiquitin conjugating enzyme E2 I, also known as SUMO-1-protein ligase, SUMO-conjugating enzyme UBC9, ubiquitin carrier protein 9, ubiquitin carrier protein Ior ubiquitin conjugating enzyme 9.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes or E1s, ubiquitin-conjugating enzymes or E2s, and ubiquitin-protein ligases or E3s. UBE2I encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for UBE2I (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE2I antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti UBE2I antibody recognizes a band of approximately 17 kDa in HT-29 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human UBE2I

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P63279
Entrez Gene
UBE2I
GO Terms
GO:0007059 chromosome segregation
GO:0000795 synaptonemal complex
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007067 mitosis
GO:0008134 transcription factor binding
GO:0016566 specific transcriptional repressor activity
GO:0016605 PML body
GO:0016925 protein sumoylation
GO:0019899 enzyme binding
GO:0043687 post-translational protein modification
GO:0044419 interspecies interaction between organisms
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0051246 regulation of protein metabolic process
GO:0051301 cell division
GO:0072500 negative regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UBE2I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml