UBE2I antibody

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Anti UBE2I Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with UBE2I antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to UBE2I (molecular weight 17 kDa)

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Rabbit anti UBE2I

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: UBE2I

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VPA00791 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human UBE2I antibody recognizes the ubiquitin conjugating enzyme E2 I, also known as SUMO-1-protein ligase, SUMO-conjugating enzyme UBC9, ubiquitin carrier protein 9, ubiquitin carrier protein Ior ubiquitin conjugating enzyme 9.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes or E1s, ubiquitin-conjugating enzymes or E2s, and ubiquitin-protein ligases or E3s. UBE2I encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for UBE2I (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE2I antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti UBE2I antibody recognizes a band of approximately 17 kDa in HT-29 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on Protein A.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide directed towards the N terminal region of human UBE2I
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P63279
Entrez Gene: UBE2I
GO Terms: GO:0007059 chromosome segregation; GO:0000795 synaptonemal complex; GO:0004842 ubiquitin-protein ligase activity; GO:0005524 ATP binding; GO:0005737 cytoplasm; GO:0006511 ubiquitin-dependent protein catabolic process; GO:0007067 mitosis; GO:0008134 transcription factor binding; GO:0016566 specific transcriptional repressor activity; View More GO Terms; GO:0016605 PML body; GO:0016925 protein sumoylation; GO:0019899 enzyme binding; GO:0043687 post-translational protein modification; GO:0044419 interspecies interaction between organisms; GO:0045892 negative regulation of transcription, DNA-dependent; GO:0051246 regulation of protein metabolic process; GO:0051301 cell division; GO:0072500 negative regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor