UBE2E2 antibody
Rabbit anti UBE2E2
Rabbit anti-human UBE2E2 antibody recognizes ubiquitin-conjugating enzyme E2 E2, also known as ubiquitin carrier protein E2 or ubiquitin-protein ligase E2. This antibody has been extensively validated for western blotting using whole cell lysates.
UBE2E2 Antibody with Control Lysate
Rabbit anti-human UBE2E2 antibody recognizes ubiquitin-conjugating enzyme E2 E2, also known as ubiquitin carrier protein E2 or ubiquitin-protein ligase E2. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00480 | WB | 100 µl | ||||
| VPA00480KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human UBE2E2 antibody recognizes UBE2E2, also known as ubiquitin carrier protein E2, ubiquitin-conjugating enzyme E2E 2, UbcH8 or ubiquitin-protein ligase E2.
Rabbit anti Human UBE2E2 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Rabbit anti Human UBE2E2 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti UBE2E2 detects a band of approximately 27 kDa in LNCaP cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the N terminal region of human UBE2E2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg LNCaP lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q96LR5 Related reagents
- Entrez Gene
- UBE2E2 Related reagents
- GO Terms
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0005730 nucleolus
- GO:0032020 ISG15-protein conjugation
- GO:0042296 ISG15 ligase activity
- GO:0043687 post-translational protein modification
- GO:0051246 regulation of protein metabolic process
- GO:0070534 protein K63-linked ubiquitination
- GO:0070936 protein K48-linked ubiquitination
- GO:0070979 protein K11-linked ubiquitination
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of UBE2E2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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