UBE2E2 antibody

100% Secure


Rabbit anti UBE2E2

<strong>Rabbit anti-human UBE2E2 antibody</strong> recognizes ubiquitin-conjugating enzyme E2 E2, also known as ubiquitin carrier protein E2 or ubiquitin-protein ligase E2. This antibody has been extensively validated for western blotting using whole cell lysates.

UBE2E2 Antibody with Control Lysate

<strong>Rabbit anti-human UBE2E2 antibody</strong> recognizes ubiquitin-conjugating enzyme E2 E2, also known as ubiquitin carrier protein E2 or ubiquitin-protein ligase E2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00480 WB 100 µl
VPA00480KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human UBE2E2 antibody recognizes UBE2E2, also known as ubiquitin carrier protein E2, ubiquitin-conjugating enzyme E2E 2, UbcH8 or ubiquitin-protein ligase E2.

Rabbit anti Human UBE2E2 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human UBE2E2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q96LR5 Related reagents
Entrez Gene
UBE2E2 Related reagents
GO Terms
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0005730 nucleolus
GO:0032020 ISG15-protein conjugation
GO:0042296 ISG15 ligase activity
GO:0043687 post-translational protein modification
GO:0051246 regulation of protein metabolic process
GO:0070534 protein K63-linked ubiquitination
GO:0070936 protein K48-linked ubiquitination
GO:0070979 protein K11-linked ubiquitination
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UBE2E2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti UBE2E2 detects a band of approximately 27 kDa in LNCaP cell lysates
Western Blotting
Anti UBE2E2 detects a band of approximately 27 kDa in LNCaP cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB