UBE2D3 antibody

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Rabbit anti UBE2D3

Rabbit anti-human UBE2D3 antibody recognizes ubiquitin-conjugating enzyme E2 D3, also known as E2(17)KB 3, PRO2116, ubiquitin carrier protein D3 or ubiquitin-protein ligase D3. This antibody has been extensively validated for western blotting using whole cell lysates.

UBE2D3 Antibody with Control Lysate

Rabbit anti-human UBE2D3 antibody recognizes ubiquitin-conjugating enzyme E2 D3, also known as E2(17)KB 3, PRO2116, ubiquitin carrier protein D3 or ubiquitin-protein ligase D3. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00461 WB 100 µl
VPA00461KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human UBE2D3 antibody recognizes the ubiquitin-conjugating enzyme E2 D3 (UBE2D3), also known as E2(17)KB 3, PRO2116, ubiquitin carrier protein D3 or ubiquitin-protein ligase D3.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D3 gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for UBE2D3, but the full-length nature of some variants has not been determined (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE2D3 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti UBE2D3 detects a band of approximately 16 kDa in NIH/3T3 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human UBE2D3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg NIH/3T3 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P61077 Related reagents
Entrez Gene
UBE2D3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000209 protein polyubiquitination
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0006281 DNA repair
GO:0006513 protein monoubiquitination
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0010008 endosome membrane
GO:0030509 BMP signaling pathway
GO:0032480 negative regulation of type I interferon production
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0043687 post-translational protein modification
GO:0051246 regulation of protein metabolic process
GO:0070936 protein K48-linked ubiquitination
GO:0070979 protein K11-linked ubiquitination
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UBE2D3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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