UBE2D3 antibody

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Anti UBE2D3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with UBE2D3 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 22 second exposure. Arrow points to UBE2D3 (molecular weight 16 kDa).

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Rabbit anti UBE2D3

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: UBE2D3

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VPA00461 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human UBE2D3 antibody recognizes the ubiquitin-conjugating enzyme E2 D3 (UBE2D3), also known as E2(17)KB 3, PRO2116, ubiquitin carrier protein D3 or ubiquitin-protein ligase D3.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D3 gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for UBE2D3, but the full-length nature of some variants has not been determined (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE2D3 antibody detects a band of 16 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti UBE2D3 detects a band of approximately 16 kDa in NIH/3T3 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the N terminal region of human UBE2D3
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P61077
Entrez Gene: UBE2D3
GO Terms: GO:0005886 plasma membrane; GO:0000209 protein polyubiquitination; GO:0005515 protein binding; GO:0006915 apoptosis; GO:0004842 ubiquitin-protein ligase activity; GO:0005524 ATP binding; GO:0006281 DNA repair; GO:0006513 protein monoubiquitination; GO:0007179 transforming growth factor beta receptor signaling pathway; View More GO Terms; GO:0010008 endosome membrane; GO:0030509 BMP signaling pathway; GO:0032480 negative regulation of type I interferon production; GO:0043161 proteasomal ubiquitin-dependent protein catabolic process; GO:0043687 post-translational protein modification; GO:0051246 regulation of protein metabolic process; GO:0070936 protein K48-linked ubiquitination; GO:0070979 protein K11-linked ubiquitination