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UBE2B antibody

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Western Blot Validated
Anti UBE2B Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with UBE2B antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to UBE2B (molecular weight 17 kDa)

Rabbit anti UBE2B

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00924
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human UBE2B antibody recognizes the ubiquitin-conjugating enzyme E2 B, also known as E2 protein, ubiquitin-conjugating enzyme E2-17 kDa, ubiquitin-conjugating enzyme E2B (RAD6 homolog), or ubiquitin-protein ligase B.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE2B antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti UBE2B antibody recognizes a band of approximately 17 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human UBE2B

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P63146
Entrez Gene
UBE2B
GO Terms
GO:0005886 plasma membrane
GO:0000209 protein polyubiquitination
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0005657 replication fork
GO:0006301 postreplication repair
GO:0006513 protein monoubiquitination
GO:0042493 response to drug
GO:0009411 response to UV
GO:0050821 protein stabilization
GO:0031625 ubiquitin protein ligase binding
GO:0033522 histone H2A ubiquitination
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0043687 post-translational protein modification
GO:0043951 negative regulation of cAMP-mediated signaling
GO:0051865 protein autoubiquitination
GO:0060070 canonical Wnt receptor signaling pathway
GO:0070534 protein K63-linked ubiquitination
GO:0070936 protein K48-linked ubiquitination
GO:0070979 protein K11-linked ubiquitination
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UBE2B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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