UBE2B antibody
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution (provided by RefSeq, Jul 2008).
Rabbit anti Human UBE2B antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti UBE2B antibody recognizes a band of approximately 17 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
<50% Glycerol - Immunogen
- Recombinant protein of human UBE2B
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P63146
- Entrez Gene
- UBE2B
- GO Terms
- GO:0005886 plasma membrane
- GO:0000209 protein polyubiquitination
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0005737 cytoplasm
- GO:0005657 replication fork
- GO:0006301 postreplication repair
- GO:0006513 protein monoubiquitination
- GO:0042493 response to drug
- GO:0009411 response to UV
- GO:0050821 protein stabilization
- GO:0031625 ubiquitin protein ligase binding
- GO:0033522 histone H2A ubiquitination
- GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
- GO:0043687 post-translational protein modification
- GO:0043951 negative regulation of cAMP-mediated signaling
- GO:0051865 protein autoubiquitination
- GO:0060070 canonical Wnt receptor signaling pathway
- GO:0070534 protein K63-linked ubiquitination
- GO:0070936 protein K48-linked ubiquitination
- GO:0070979 protein K11-linked ubiquitination
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of UBE2B antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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