UBE2B antibody

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Rabbit anti UBE2B

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00924 Datasheet	WB SDS	100 µl	Log in		Get Quote

Rabbit anti Human UBE2B antibody recognizes the ubiquitin-conjugating enzyme E2 B, also known as E2 protein, ubiquitin-conjugating enzyme E2-17 kDa, ubiquitin-conjugating enzyme E2B (RAD6 homolog), or ubiquitin-protein ligase B.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution (provided by RefSeq, Jul 2008).

Rabbit anti Human UBE2B antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti UBE2B antibody recognizes a band of approximately 17 kDa in HeLa cell lysates
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
<50% Glycerol
Immunogen: Recombinant protein of human UBE2B

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee: 12 months from date of despatch

More Information

UniProt: P63146
Entrez Gene: UBE2B
GO Terms: GO:0005886 plasma membrane; GO:0000209 protein polyubiquitination; GO:0004842 ubiquitin-protein ligase activity; GO:0005524 ATP binding; GO:0005737 cytoplasm; GO:0005657 replication fork; GO:0006301 postreplication repair; GO:0006513 protein monoubiquitination; GO:0042493 response to drug; GO:0009411 response to UV; GO:0050821 protein stabilization; GO:0031625 ubiquitin protein ligase binding; GO:0033522 histone H2A ubiquitination; GO:0043161 proteasomal ubiquitin-dependent protein catabolic process; GO:0043687 post-translational protein modification; GO:0043951 negative regulation of cAMP-mediated signaling; GO:0051865 protein autoubiquitination; GO:0060070 canonical Wnt receptor signaling pathway; GO:0070534 protein K63-linked ubiquitination; GO:0070936 protein K48-linked ubiquitination; GO:0070979 protein K11-linked ubiquitination
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of UBE2B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in		Get Quote

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