UBE2B antibody
Rabbit anti Human UBE2B antibody recognizes the ubiquitin-conjugating enzyme E2 B, also known as E2 protein, ubiquitin-conjugating enzyme E2-17 kDa, ubiquitin-conjugating enzyme E2B (RAD6 homolog), or ubiquitin-protein ligase B.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution (provided by RefSeq, Jul 2008).
Rabbit anti Human UBE2B antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution (provided by RefSeq, Jul 2008).
Rabbit anti Human UBE2B antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti UBE2B antibody recognizes a band of approximately 17 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
<50% Glycerol - Immunogen
- Recombinant protein of human UBE2B
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P63146 Related reagents
- Entrez Gene
- UBE2B Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0000209 protein polyubiquitination
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0005737 cytoplasm
- GO:0005657 replication fork
- GO:0006301 postreplication repair
- GO:0006513 protein monoubiquitination
- GO:0042493 response to drug
- GO:0009411 response to UV
- GO:0050821 protein stabilization
- GO:0031625 ubiquitin protein ligase binding
- GO:0033522 histone H2A ubiquitination
- GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
- GO:0043687 post-translational protein modification
- GO:0043951 negative regulation of cAMP-mediated signaling
- GO:0051865 protein autoubiquitination
- GO:0060070 canonical Wnt receptor signaling pathway
- GO:0070534 protein K63-linked ubiquitination
- GO:0070936 protein K48-linked ubiquitination
- GO:0070979 protein K11-linked ubiquitination
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of UBE2B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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