UBE2 L3 antibody

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Goat anti UBE2 L3

UBE2L3 antibody recognizes ubiquitin-conjugating enzyme E2 L3, also known as L-UBC, UbcH7, ubiquitin carrier protein L3, ubiquitin-conjugating enzyme E2-F1 or ubiquitin-protein ligase L3. The antibody has been extensively validated for western blotting using whole cell lysates.

UBE2 L3 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size
VPA00111	WB	100 µl
VPA00111KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Goat anti Human UBE2 L3 antibody recognizes the ubiquitin-conjugating enzyme E2 L3, also known as L-UBC, UbcH7, ubiquitin carrier protein L3, ubiquitin-conjugating enzyme E2-F1 or ubiquitin-protein ligase L3.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). Encloded by the UBE2L3 gene, ubiquitin-conjugating enzyme E2 L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for UBE2L3 (provided by RefSeq, Sep 2009).

Goat anti Human UBE2 L3 antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Goat polyclonal antibody purified by affinity chromatography.

Preparation

20μl Goat polyclonal antibody purified by affinity chromatography

Buffer Solution

TRIS buffered saline

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃) 0.5 % BSA

Preservative Stabilisers

0.02% Sodium Azide (NaN₃) 0.5 % BSA

Immunogen

Peptide with the sequence C-EFTKKYGEKRPVD, from the C Terminus of the protein sequence.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P68036 Related reagents
Entrez Gene: UBE2L3 Related reagents
GO Terms: GO:0000151 ubiquitin ligase complex; GO:0000209 protein polyubiquitination; GO:0003713 transcription coactivator activity; GO:0004842 ubiquitin-protein ligase activity; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0006511 ubiquitin-dependent protein catabolic process; GO:0007090 regulation of S phase of mitotic cell cycle; GO:0008283 cell proliferation; GO:0019899 enzyme binding; GO:0032583 regulation of gene-specific transcription; GO:0043687 post-translational protein modification; GO:0051246 regulation of protein metabolic process; GO:0070979 protein K11-linked ubiquitination; GO:0071385 cellular response to glucocorticoid stimulus
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of UBE2 L3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti UBE2 L3 detects a band of approximately 18 kDa in K562 cell lysates.
Western Blotting: Anti UBE2 L3 detects a band of approximately 18 kDa in K562 cell lysates.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB

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