UBE2 L3 antibody

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Goat anti UBE2 L3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00111
Datasheet
WB
MSDS
100 µl

Goat anti Human UBE2 L3 antibody recognizes the ubiquitin-conjugating enzyme E2 L3, also known as L-UBC, UbcH7, ubiquitin carrier protein L3, ubiquitin-conjugating enzyme E2-F1 or ubiquitin-protein ligase L3.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). Encloded by the UBE2L3 gene, ubiquitin-conjugating enzyme E2 L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for UBE2L3 (provided by RefSeq, Sep 2009).

Goat anti Human UBE2 L3 antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti UBE2 L3 detects a band of approximately 18 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-EFTKKYGEKRPVD, from the C Terminus of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P68036
Entrez Gene
UBE2L3
GO Terms
GO:0000151 ubiquitin ligase complex
GO:0000209 protein polyubiquitination
GO:0003713 transcription coactivator activity
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007090 regulation of S phase of mitotic cell cycle
GO:0008283 cell proliferation
GO:0019899 enzyme binding
GO:0032583 regulation of gene-specific transcription
GO:0043687 post-translational protein modification
GO:0051246 regulation of protein metabolic process
GO:0070979 protein K11-linked ubiquitination
GO:0071385 cellular response to glucocorticoid stimulus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of UBE2 L3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader