UBE2 L3 antibody
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WB ResetGoat anti UBE2 L3
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- UBE2 L3
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| Goat anti Human UBE2 L3 antibody recognizes the ubiquitin-conjugating enzyme E2 L3, also known as L-UBC, UbcH7, ubiquitin carrier protein L3, ubiquitin-conjugating enzyme E2-F1 or ubiquitin-protein ligase L3. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). Encloded by the UBE2L3 gene, ubiquitin-conjugating enzyme E2 L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for UBE2L3 (provided by RefSeq, Sep 2009). Goat anti Human UBE2 L3 antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti UBE2 L3 detects a band of approximately 18 kDa in K562 cell lysates.
- Species Cross-Reactivity
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Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5 % BSA
- Immunogen
- Peptide with the sequence C-EFTKKYGEKRPVD, from the C Terminus of the protein sequence.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Donkey anti Sheep/Goat IgG:HRP | ||||||
- UniProt
- P68036
- Entrez Gene
- UBE2L3
- GO Terms
- GO:0000151 ubiquitin ligase complex
- GO:0000209 protein polyubiquitination
- GO:0003713 transcription coactivator activity
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006511 ubiquitin-dependent protein catabolic process
- GO:0007090 regulation of S phase of mitotic cell cycle
- View More GO Terms
- GO:0008283 cell proliferation
- GO:0019899 enzyme binding
- GO:0032583 regulation of gene-specific transcription
- GO:0043687 post-translational protein modification
- GO:0051246 regulation of protein metabolic process
- GO:0070979 protein K11-linked ubiquitination
- GO:0071385 cellular response to glucocorticoid stimulus
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