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|Rabbit anti Human UBC13 antibody recognizes UBC13, also known as ubiquitin-conjugating enzyme E2 N (UBE2N), an important component of the toll-like receptor and interleukin-1R signalling pathway.
UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Martein et al. 2009).
Rabbit anti Human UBC13 antibody detects ubiquitin-conjugating enzyme E2 N as a band of 17 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
- Target Species
- Western Blotting
- Rabbit anti UBC13 detects a band of approximately 17 kDa in K562 cell lysates.
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- A 15 amino acid peptide located near the human UBC13 C-terminus.
- For research purposes only.
- 12 months from date of despatch.
- PrecisionAb is a trademark of Bio-Rad Laboratories.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
References for UBC13 antibody
Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
J Cell Biol. 186 (5): 685-92.
Burr, M.L. et al. (2013) MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum luminal residues during HRD1 ubiquitin E3 ligase-mediated dislocation.
Proc Natl Acad Sci U S A. 110 (35): 14290-5.
Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway.
Nat Cell Biol. 7 (8): 758-65.
- Entrez Gene
- GO Terms
- GO:0000724 double-strand break repair via homologous recombination
- GO:0000729 DNA double-strand break processing
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006301 postreplication repair
- GO:0006508 proteolysis
- View More GO Terms
- GO:0008063 Toll signaling pathway
- GO:0016574 histone ubiquitination
- GO:0031058 positive regulation of histone modification
- GO:0031372 UBC13-MMS2 complex
- GO:0033182 regulation of histone ubiquitination
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043130 ubiquitin binding
- GO:0043687 post-translational protein modification
- GO:0045087 innate immune response
- GO:0045739 positive regulation of DNA repair
- GO:0050852 T cell receptor signaling pathway
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051443 positive regulation of ubiquitin-protein ligase activity
- GO:0070534 protein K63-linked ubiquitination
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