UBC13 antibody
Rabbit anti UBC13
UBC13 antibody recognizes ubiquitin-conjugating enzyme E2 N, also known as bendless-like ubiquitin-conjugating enzyme, UBC13, UBCH13, ubiquitin carrier protein N or ubiquitin-protein ligase N. The antibody has been extensively validated for western blotting using whole cell lysates.
UBC13 Antibody with Control Lysate
UBC13 antibody recognizes ubiquitin-conjugating enzyme E2 N, also known as bendless-like ubiquitin-conjugating enzyme, UBC13, UBCH13, ubiquitin carrier protein N or ubiquitin-protein ligase N. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00090 | WB | 100 µl | ||||
| VPA00090KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human UBC13 antibody recognizes UBC13, also known as ubiquitin-conjugating enzyme E2 N (UBE2N), an important component of the toll-like receptor and interleukin-1R signalling pathway.
UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Martein et al. 2009).
Rabbit anti Human UBC13 antibody detects ubiquitin-conjugating enzyme E2 N as a band of 17 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Martein et al. 2009).
Rabbit anti Human UBC13 antibody detects ubiquitin-conjugating enzyme E2 N as a band of 17 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti UBC13 detects a band of approximately 17 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- A 15 amino acid peptide located near the human UBC13 C-terminus.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P61088 Related reagents
- Entrez Gene
- UBE2N Related reagents
- GO Terms
- GO:0000724 double-strand break repair via homologous recombination
- GO:0000729 DNA double-strand break processing
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006301 postreplication repair
- GO:0006508 proteolysis
- GO:0008063 Toll signaling pathway
- GO:0016574 histone ubiquitination
- GO:0031058 positive regulation of histone modification
- GO:0031372 UBC13-MMS2 complex
- GO:0033182 regulation of histone ubiquitination
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043130 ubiquitin binding
- GO:0043687 post-translational protein modification
- GO:0045087 innate immune response
- GO:0045739 positive regulation of DNA repair
- GO:0050852 T cell receptor signaling pathway
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051443 positive regulation of ubiquitin-protein ligase activity
- GO:0070534 protein K63-linked ubiquitination
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of UBC13 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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