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UBC13 antibody

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Anti UBC13 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti UBC13 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2
Anti UBC13 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Rabbit anti Human UBC13 antibody (VPA00090) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to UBC13 (molecular weight 17 kDa).
Anti UBC13 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Immunohistochemical staining of mouse thymus with Rabbit anti Mouse Ubc13 (VPA00090).

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Rabbit anti UBC13

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
UBC13

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Product Code Applications Pack Size List Price Your Price Qty
VPA00090
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Rabbit anti Human UBC13 antibody recognizes UBC13, also known as ubiquitin-conjugating enzyme E2 N (UBE2N), an important component of the toll-like receptor and interleukin-1R signalling pathway.

UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Martein et al. 2009).

Rabbit anti Human UBC13 antibody detects ubiquitin-conjugating enzyme E2 N as a band of 17 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti UBC13 detects a band of approximately 17 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
A 15 amino acid peptide located near the human UBC13 C-terminus.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

References for UBC13 antibody

  1. Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
    J Cell Biol. 186 (5): 685-92.
  2. Burr, M.L. et al. (2013) MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum luminal residues during HRD1 ubiquitin E3 ligase-mediated dislocation.
    Proc Natl Acad Sci U S A. 110 (35): 14290-5.

Further Reading

  1. Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway.
    Nat Cell Biol. 7 (8): 758-65.

Western Blotting

UniProt
P61088
Entrez Gene
UBE2N
GO Terms
GO:0000724 double-strand break repair via homologous recombination
GO:0000729 DNA double-strand break processing
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006301 postreplication repair
GO:0006508 proteolysis
GO:0008063 Toll signaling pathway
GO:0016574 histone ubiquitination
GO:0031058 positive regulation of histone modification
GO:0031372 UBC13-MMS2 complex
GO:0033182 regulation of histone ubiquitination
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043130 ubiquitin binding
GO:0043687 post-translational protein modification
GO:0045087 innate immune response
GO:0045739 positive regulation of DNA repair
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051443 positive regulation of ubiquitin-protein ligase activity
GO:0070534 protein K63-linked ubiquitination
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