Rabbit anti Human UBC13 antibody
recognizes the C-terminal (CT) region of Ubiquitin-conjugating enzyme E2N (UBC13), also known as Bendless-like ubiquitin-conjugating enzyme, Ubiquitin carrier protein N or Ubiquitin-protein ligase N. UBC13 is a ~16kDa enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway.
UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Martein et al. 2009
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human UBC13 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- A 15 amino acid peptide located near the human UBC13 carboxy-terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
double-strand break repair via homologous recombination
DNA double-strand break processing
MyD88-dependent toll-like receptor signaling pathway
ubiquitin-protein ligase activity
Toll signaling pathway
positive regulation of histone modification
regulation of histone ubiquitination
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 4 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB cascade
post-translational protein modification
innate immune response
positive regulation of DNA repair
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of ubiquitin-protein ligase activity
protein K63-linked ubiquitination
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of UBC13 antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Mouse thymus tissue
- Western Blotting
- Rabbit anti human UBC13 antibody detects a band of ~16kDa in human small intestine cell lystates.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for UBC13 antibody
Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
J Cell Biol. 186: 685-92.
Burr, M.L. et al. (2013) MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum luminal residues during HRD1 ubiquitin E3 ligase-mediated dislocation.
Proc Natl Acad Sci U S A. 110 (35): 14290-5.
Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway.
Nat Cell Biol. 7 (8): 758-65.
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