UBC13 antibody

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Rabbit anti UBC13 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP974
Datasheet
P* WB
MSDS
0.1 mg loader

Rabbit anti Human UBC13 antibody recognizes the C-terminal (CT) region of Ubiquitin-conjugating enzyme E2N (UBC13), also known as Bendless-like ubiquitin-conjugating enzyme, Ubiquitin carrier protein N or Ubiquitin-protein ligase N. UBC13 is a ~16kDa enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway.

UBC13 is a DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation (Martein et al. 2009).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human UBC13 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 15 amino acid peptide located near the human UBC13 carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P61088 Related reagents
Entrez Gene
UBE2N Related reagents
GO Terms
GO:0000724 double-strand break repair via homologous recombination
GO:0000729 DNA double-strand break processing
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006301 postreplication repair
GO:0006508 proteolysis
GO:0008063 Toll signaling pathway
GO:0016574 histone ubiquitination
GO:0031058 positive regulation of histone modification
GO:0031372 UBC13-MMS2 complex
GO:0033182 regulation of histone ubiquitination
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043130 ubiquitin binding
GO:0043687 post-translational protein modification
GO:0045087 innate immune response
GO:0045739 positive regulation of DNA repair
GO:0050852 T cell receptor signaling pathway
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051443 positive regulation of ubiquitin-protein ligase activity
GO:0070534 protein K63-linked ubiquitination
Regulatory
For research purposes only

Applications of UBC13 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2ug/ml
Western Blotting 0.5ug/ml 1ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse thymus tissue
Western Blotting
Rabbit anti human UBC13 antibody detects a band of ~16kDa in human small intestine cell lystates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Western Blotting

Product Specific References

References for UBC13 antibody

  1. Stagg, H.R. et al. (2009) The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.
    J Cell Biol. 186: 685-92.
  2. Burr, M.L. et al. (2013) MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum luminal residues during HRD1 ubiquitin E3 ligase-mediated dislocation.
    Proc Natl Acad Sci U S A. 110 (35): 14290-5.

Further Reading

  1. Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway.
    Nat Cell Biol. 7 (8): 758-65.
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