UBA1 antibody

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Rabbit anti UBA1

UBA1 antibody recognizes ubiquitin-like modifier-activating enzyme 1, also known as A1S9T and BN75 temperature sensitivity complementing, POC20 centriolar protein homolog, or ubiquitin-activating enzyme E1 homolog A.

UBA1 Antibody with Control Lysate

UBA1 antibody recognizes ubiquitin-like modifier-activating enzyme 1, also known as A1S9T and BN75 temperature sensitivity complementing, POC20 centriolar protein homolog, or ubiquitin-activating enzyme E1 homolog A.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00195 WB 100 µl
VPA00195KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human UBA1 antibody recognizes UBA1, also known as A1S9T and BN75 temperature sensitivity complementing, POC20 centriolar protein homolog, ubiquitin-activating enzyme E1 homolog A or ubiquitin-like modifier-activating enzyme 1.

The protein encoded by the UBA1 gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. UBA1 complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described (provided by RefSeq, Jul 2008).

Rabbit anti Human UBA1 antibody detects a band of 118 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by ion exchange chromatography.
Preparation
20μl Rabbit polyclonal antibody purified by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P22314 Related reagents
Entrez Gene
UBA1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0006464 protein modification process
GO:0008219 cell death
GO:0008641 small protein activating enzyme activity
GO:0016874 ligase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of UBA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti UBA1 detects a band of approximately 118 kDa in HEK293 cell lysates.
Western Blotting
Anti UBA1 detects a band of approximately 118 kDa in HEK293 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB